Please use this identifier to cite or link to this item: https://doi.org/10.1002/jbm.a.32899
Title: TGF-β3 immobilized PLGA-gelatin/chondroitin sulfate/hyaluronic acid hybrid scaffold for cartilage regeneration
Authors: Fan, H.
Tao, H.
Wu, Y. 
Hu, Y.
Yan, Y.
Luo, Z.
Keywords: cartilage
mesenchymal stem cells
tissue engineering
transforming growth factor
Issue Date: 15-Dec-2010
Citation: Fan, H., Tao, H., Wu, Y., Hu, Y., Yan, Y., Luo, Z. (2010-12-15). TGF-β3 immobilized PLGA-gelatin/chondroitin sulfate/hyaluronic acid hybrid scaffold for cartilage regeneration. Journal of Biomedical Materials Research - Part A 95 (4) : 982-992. ScholarBank@NUS Repository. https://doi.org/10.1002/jbm.a.32899
Abstract: Although most in vitro studies indicate that transforming growth factor β3 (TGF-β3) immobilized scaffold is suitable for cartilage tissue engineering, in vivo studies of implanting immobilized scaffold for chondral defect repair are still lacking. This study is to evaluate the potentials of TGF-β3 immobilized poly-(lactic-co-glycolic acid)-gelatin/chondroitin sulfate/hyaluronic acid (PLGA-GCH) hybrid scaffold for cartilage regeneration. The scaffold was fabricated by incorporating GCH micro-sponges into PLGA frameworks and then crosslinked with TGF-β3 to mimic natural cartilaginous extra cellular matrix (ECM). In vitro study demonstrated that MSCs proliferated vigorously and produced abundant ECM on scaffold. The immunohistochemistry staining and alcian blue staining confirmed the cartilaginous ECM production. The chondrogenic differentiation of MSCs on scaffold was proved by the expression of collagen II gene in mRNA and protein level. Then MSCs/TGF-β3 immobilized scaffolds were implanted in rabbits for chondral defects repair. After eight weeks, histological observation showed that differentiated MSCs were located in lacunae within the metachromatic staining matrix and exhibited typical chondrocyte morphology. Histological grading scores also indicated the congruent cartilage was regenerated. In conclusion, the TGF-β3 immobilized PLGA-GCH hybrid scaffold has great potential in constructing the tissue-engineered cartilage. © 2010 Wiley Periodicals, Inc.
Source Title: Journal of Biomedical Materials Research - Part A
URI: http://scholarbank.nus.edu.sg/handle/10635/109684
ISSN: 15493296
DOI: 10.1002/jbm.a.32899
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

81
checked on Nov 23, 2022

WEB OF SCIENCETM
Citations

70
checked on Nov 23, 2022

Page view(s)

179
checked on Nov 24, 2022

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.