Please use this identifier to cite or link to this item: https://doi.org/10.1007/s00432-011-1115-9
Title: Reliability of tumor primary cultures as a model for drug response prediction: Expression profiles comparison of tissues versus primary cultures from colorectal cancer patients
Authors: Bellot, G.L. 
Tan, W.H.
Tay, L.L. 
Koh, D.
Wang, X.
Keywords: Colorectal cancer
Drug assays
Drug resistance
Microarray
Primary cultures
Issue Date: Mar-2012
Citation: Bellot, G.L., Tan, W.H., Tay, L.L., Koh, D., Wang, X. (2012-03). Reliability of tumor primary cultures as a model for drug response prediction: Expression profiles comparison of tissues versus primary cultures from colorectal cancer patients. Journal of Cancer Research and Clinical Oncology 138 (3) : 463-482. ScholarBank@NUS Repository. https://doi.org/10.1007/s00432-011-1115-9
Abstract: Purpose: Since primary tumor cells from patients have been used as a model for assessment of drug response for individual patients, this study aims to evaluate the reliability of such a model in colorectal cancer (CRC) in predicting the response of tumor tissues through comparison of their expression profiles. Methods: Establishment of primary cultures from tissues obtained surgically from CRC patients allowed us to study the gene expression differences between normal and tumor tissues as well as primary cultures derived from the tumor mass. The tissues comparison highlights the molecular characteristics of tumors, while the comparison between primary tumor cells versus normal and tumor tissues allowed us to identify alterations associated with the establishment of culture. Genes-drug association analyses allowed us to fine-tune our expectations while using primary culture as a model for drug assessment. Results: Comparison between tumor cultures and original tissues through functional analyses showed the deregulations caused by culture establishment. Investigating the impact of such changes in genes-drug associations to identify the potential alterations in drug response, we found that primary cultures may have increased susceptibility toward paclitaxel, but reduced susceptibility toward analogues of Xuorouracil compared with original tumors. Conclusions: Response of primary tumor cells toward different drugs is not linearly associated to tumor tissues. Our results highlight the importance to account for the discrepancy in responses between the primary tumor cells and original counterparts in order to provide clinicians with important insights to improve selection of drugs for individual patients based on in vitro assays. © Springer-Verlag 2011.
Source Title: Journal of Cancer Research and Clinical Oncology
URI: http://scholarbank.nus.edu.sg/handle/10635/109588
ISSN: 01715216
DOI: 10.1007/s00432-011-1115-9
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

6
checked on Jun 30, 2022

WEB OF SCIENCETM
Citations

6
checked on Jun 30, 2022

Page view(s)

140
checked on Jun 23, 2022

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.