Please use this identifier to cite or link to this item: https://doi.org/10.1007/s11010-013-1776-6
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dc.titleNogo/RTN4 isoforms and RTN3 expression protect SH-SY5Y cells against multiple death insults
dc.contributor.authorTeng, F.Y.H.
dc.contributor.authorTang, B.L.
dc.date.accessioned2014-11-26T07:46:25Z
dc.date.available2014-11-26T07:46:25Z
dc.date.issued2013-12
dc.identifier.citationTeng, F.Y.H., Tang, B.L. (2013-12). Nogo/RTN4 isoforms and RTN3 expression protect SH-SY5Y cells against multiple death insults. Molecular and Cellular Biochemistry 384 (1-2) : 7-19. ScholarBank@NUS Repository. https://doi.org/10.1007/s11010-013-1776-6
dc.identifier.issn03008177
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/109489
dc.description.abstractAmong the members of the reticulon (RTN) family, Nogo-A/RTN4A, a prominent myelin-associated neurite growth inhibitory protein, and RTN3 are highly expressed in neurons. However, neuronal cell-autonomous functions of Nogo-A, as well as other members of the RTN family, are unclear. We show here that SH-SY5Y neuroblastoma cells stably over-expressing either two of the three major isoforms of Nogo/RTN4 (Nogo-A and Nogo-B) or a major isoform of RTN3 were protected against cell death induced by a battery of apoptosis-inducing agents (including serum deprivation, staurosporine, etoposide, and H2O 2) compared to vector-transfected control cells. Nogo-A, -B, and RTN3 are particularly effective in terms of protection against H2O 2-induced increase in intracellular reactive oxygen species levels and ensuing apoptotic and autophagic cell death. Expression of these RTNs upregulated basal levels of Bax, activated Bax, and activated caspase 3, but did not exhibit an enhanced ER stress response. The protective effect of RTNs is also not dependent on classical survival-promoting signaling pathways such as Akt and Erk kinase pathways. Neuron-enriched Nogo-A/Rtn4A and RTN3 may, therefore, exert a protective effect on neuronal cells against death stimuli, and elevation of their levels during injury may have a cell-autonomous survival-promoting function. © 2013 Springer Science+Business Media New York.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1007/s11010-013-1776-6
dc.sourceScopus
dc.subjectCNS injury
dc.subjectNeuroprotection
dc.subjectNogo
dc.subjectReticulon 3 (RTN3)
dc.typeArticle
dc.contributor.departmentBIOCHEMISTRY
dc.description.doi10.1007/s11010-013-1776-6
dc.description.sourcetitleMolecular and Cellular Biochemistry
dc.description.volume384
dc.description.issue1-2
dc.description.page7-19
dc.description.codenMCBIB
dc.identifier.isiut000326263500002
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