Please use this identifier to cite or link to this item:
|Title:||Limited gene expression variation in human embryonic stem cell and induced pluripotent stem cell-derived endothelial cells||Authors:||White, M.P.
Induced pluripotent stem cells
|Issue Date:||Jan-2013||Citation:||White, M.P., Rufaihah, A.J., Liu, L., Ghebremariam, Y.T., Ivey, K.N., Cooke, J.P., Srivastava, D. (2013-01). Limited gene expression variation in human embryonic stem cell and induced pluripotent stem cell-derived endothelial cells. Stem Cells 31 (1) : 92-103. ScholarBank@NUS Repository. https://doi.org/10.1002/stem.1267||Abstract:||Recent evidence suggests human embryonic stem cell (hESC) and induced pluripotent stem (iPS) cell lines have differences in their epigenetic marks and transcriptomes, yet the impact of these differences on subsequent terminally differentiated cells is less well understood. Comparison of purified, homogeneous populations of somatic cells derived from multiple independent human iPS and ES lines will be required to address this critical question. Here, we report a differentiation protocol based on embryonic development that consistently yields large numbers of endothelial cells (ECs) derived from multiple hESCs or iPS cells. Mesoderm differentiation of embryoid bodies was maximized, and defined growth factors were used to generate KDR+ EC progenitors. Magnetic purification of a KDR+ progenitor subpopulation resulted in an expanding, homogeneous pool of ECs that expressed EC markers and had functional properties of ECs. Comparison of the transcriptomes revealed limited gene expression variability between multiple lines of human iPS-derived ECs or between lines of ES- and iPS-derived ECs. These results demonstrate a method to generate large numbers of pure human EC progenitors and differentiated ECs from pluripotent stem cells and suggest individual lineages derived from human iPS cells may have significantly less variance than their pluripotent founders. © AlphaMed Press.||Source Title:||Stem Cells||URI:||http://scholarbank.nus.edu.sg/handle/10635/109436||ISSN:||10665099||DOI:||10.1002/stem.1267|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Aug 6, 2020
WEB OF SCIENCETM
checked on Aug 6, 2020
checked on Aug 1, 2020
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.