Please use this identifier to cite or link to this item:
https://doi.org/10.1074/jbc.M111.265207
DC Field | Value | |
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dc.title | Functional characterization of alternative splicing in the C terminus of L-type Ca v1.3 channels | |
dc.contributor.author | Tan, B.Z. | |
dc.contributor.author | Jiang, F. | |
dc.contributor.author | Tan, M.Y. | |
dc.contributor.author | Yu, D. | |
dc.contributor.author | Huang, H. | |
dc.contributor.author | Shen, Y. | |
dc.contributor.author | Soong, T.W. | |
dc.date.accessioned | 2014-11-26T07:44:48Z | |
dc.date.available | 2014-11-26T07:44:48Z | |
dc.date.issued | 2011-12-09 | |
dc.identifier.citation | Tan, B.Z., Jiang, F., Tan, M.Y., Yu, D., Huang, H., Shen, Y., Soong, T.W. (2011-12-09). Functional characterization of alternative splicing in the C terminus of L-type Ca v1.3 channels. Journal of Biological Chemistry 286 (49) : 42725-42735. ScholarBank@NUS Repository. https://doi.org/10.1074/jbc.M111.265207 | |
dc.identifier.issn | 00219258 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/109354 | |
dc.description.abstract | Ca V1.3 channels are unique among the high voltage-activated Ca 2+ channel family because they activate at the most negative potentials and display very rapid calcium-dependent inactivation. Both properties are of crucial importance in neurons of the suprachiasmatic nucleus and substantia nigra, where the influx of Ca 2+ ions at subthreshold membrane voltages supports pacemaking function. Previously, alternative splicing in the Ca V1.3C terminus gives rise to a long (Ca V1.3 42) and a short form (Ca V1.3 42A), resulting in a pronounced activation at more negative voltages and faster inactivation in the latter. It was further shown that the C-terminal modulator in the Ca V1.3 42 isoforms modulates calmodulin binding to the IQ domain. Using splice variant-specific antibodies, we determined that protein localization of both splice variants in different brain regions were similar. Using the transcript-scanning method, we further identified alternative splicing at four loci in theCterminus of Ca V1.3 channels. Alternative splicing of exon 41 removes the IQ motif, resulting in a truncated Ca V1.3 protein with diminished inactivation. Splicing of exon 43 causes a frameshift and exhibits a robust inactivation of similar intensity to Ca V1.3 42A. Alternative splicing of exons 44 and 48 are in-frame, altering interaction of the distal modulator with the IQ domain and tapering inactivation slightly. Thus, alternative splicing in the C terminus of Ca V1.3 channels modulates its electrophysiological properties, which could in turn alter neuronal firing properties and functions. © 2011 by The American Society for Biochemistry and Molecular Biology, Inc. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1074/jbc.M111.265207 | |
dc.source | Scopus | |
dc.type | Article | |
dc.contributor.department | PHYSIOLOGY | |
dc.description.doi | 10.1074/jbc.M111.265207 | |
dc.description.sourcetitle | Journal of Biological Chemistry | |
dc.description.volume | 286 | |
dc.description.issue | 49 | |
dc.description.page | 42725-42735 | |
dc.description.coden | JBCHA | |
dc.identifier.isiut | 000298180900079 | |
Appears in Collections: | Staff Publications |
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