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Title: CDK regulates septin organization through cell-cycle-dependent phosphorylation of the Nim1-related kinase Gin4
Authors: Li, C.-R.
Yong, J.-Y.A.
Wang, Y.-M.
Wang, Y. 
Keywords: Candida albicans
Cell cycle
Cyclin-dependent kinase
Nim1-related kinase
Issue Date: 2012
Citation: Li, C.-R., Yong, J.-Y.A., Wang, Y.-M., Wang, Y. (2012). CDK regulates septin organization through cell-cycle-dependent phosphorylation of the Nim1-related kinase Gin4. Journal of Cell Science 125 (10) : 2533-2543. ScholarBank@NUS Repository.
Abstract: Cyclin-dependent kinases (CDKs) regulate septin organization in a cell-cycle-dependent manner in yeast. However, the mechanism remains unclear. Here, we show that the Candida albicans CDK Cdc28 phosphorylates the Nim1-related kinase Gin4, a known septin regulator, activating its kinase activity, which in turn phosphorylates the Sep7 septin. Gin4 contains a cluster of CDK phosphorylation sites near the kinase domain. Replacing serine/threonine with alanine in these sites prevents Gin4 activation, weakens its association with Sep7, alters Sep7 dynamics and causes morphological and cytokinetic defects. By contrast, phosphomimetic mutation enhances the kinase activity with only moderate deteriorating effects. We also found that Gin4 has both kinase-independent and -dependent functions, acting during G1 phase and mitosis, respectively, with the former being essential for septin ring assembly. Thus, we have identified a previously unknown signaling pathway linking CDKs and the septins that provides new insights into the mechanisms controlling septin organization and function in coordination with cell-cycle phases. © 2012. Published by The Company of Biologists Ltd.
Source Title: Journal of Cell Science
ISSN: 00219533
DOI: 10.1242/jcs.104497
Appears in Collections:Staff Publications

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