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|Title:||Autocrine proliferative effects of hGH are maintained in primary cultures of human mammary carcinoma cells||Authors:||Chiesa, J.
|Issue Date:||Sep-2011||Citation:||Chiesa, J., Ferrer, C., Arnould, C., Vouyovitch, C.M., Diaz, J.-J., Gonzalez, S., Mares, P., Morel, G., Wu, Z.-S., Zhu, T., Lobie, P.E., Mertani, H.C. (2011-09). Autocrine proliferative effects of hGH are maintained in primary cultures of human mammary carcinoma cells. Journal of Clinical Endocrinology and Metabolism 96 (9) : E1418-E1426. ScholarBank@NUS Repository. https://doi.org/10.1210/jc.2011-0473||Abstract:||Context: Empirical evidence suggests that autocrine human GH (hGH) may possess a proliferative and oncogenic role in human mammary carcinoma. However, this concept is largely derived from studies using cultured human mammary carcinoma cell (HMCC) lines. Objective: We investigated the expression and functionality of hGH and the hGH receptor in isolated cultures of primary HMCC. Design: Epithelial cell adhesion molecule-positive primary HMCC were isolated from surgical biopsies of patients with mammary carcinoma and cultured in vitro. Expression of hGH and hGH receptor was determined by RT-PCR, immunofluorescence microscopy, and ELISA. The proliferative response of the cultured primary HMCC to hGH stimulation or hGH inhibition with a hGH antagonist was determined. Results: One hundred percent of cultured primary HMCC expressed the hGH receptor, and 52% expressed hGH at the mRNA level. hGH-positive primary HMCC produced hGH protein within the cell and secreted hGH to the media. Both hGH-negative and hGH-positive HMCC responded to hGH stimulation with large increases in cell number. hGH-positive HMCC responded to inhibition of hGH by a hGH antagonist with a decrease in cell number, whereas hGH-negative HMCC did not. Conclusion: Primary HMCC proliferate in response to hGH, and the proliferation of hGH-positive HMCC is inhibited by hGH antagonism. Inhibition of hGH in patients with mammary carcinoma may therefore limit tumor growth. Copyright © 2011 by The Endocrine Society.||Source Title:||Journal of Clinical Endocrinology and Metabolism||URI:||http://scholarbank.nus.edu.sg/handle/10635/109209||ISSN:||0021972X||DOI:||10.1210/jc.2011-0473|
|Appears in Collections:||Staff Publications|
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