Please use this identifier to cite or link to this item:
Title: Autocrine proliferative effects of hGH are maintained in primary cultures of human mammary carcinoma cells
Authors: Chiesa, J.
Ferrer, C.
Arnould, C.
Vouyovitch, C.M.
Diaz, J.-J.
Gonzalez, S.
Mares, P.
Morel, G.
Wu, Z.-S.
Zhu, T.
Lobie, P.E. 
Mertani, H.C.
Issue Date: Sep-2011
Citation: Chiesa, J., Ferrer, C., Arnould, C., Vouyovitch, C.M., Diaz, J.-J., Gonzalez, S., Mares, P., Morel, G., Wu, Z.-S., Zhu, T., Lobie, P.E., Mertani, H.C. (2011-09). Autocrine proliferative effects of hGH are maintained in primary cultures of human mammary carcinoma cells. Journal of Clinical Endocrinology and Metabolism 96 (9) : E1418-E1426. ScholarBank@NUS Repository.
Abstract: Context: Empirical evidence suggests that autocrine human GH (hGH) may possess a proliferative and oncogenic role in human mammary carcinoma. However, this concept is largely derived from studies using cultured human mammary carcinoma cell (HMCC) lines. Objective: We investigated the expression and functionality of hGH and the hGH receptor in isolated cultures of primary HMCC. Design: Epithelial cell adhesion molecule-positive primary HMCC were isolated from surgical biopsies of patients with mammary carcinoma and cultured in vitro. Expression of hGH and hGH receptor was determined by RT-PCR, immunofluorescence microscopy, and ELISA. The proliferative response of the cultured primary HMCC to hGH stimulation or hGH inhibition with a hGH antagonist was determined. Results: One hundred percent of cultured primary HMCC expressed the hGH receptor, and 52% expressed hGH at the mRNA level. hGH-positive primary HMCC produced hGH protein within the cell and secreted hGH to the media. Both hGH-negative and hGH-positive HMCC responded to hGH stimulation with large increases in cell number. hGH-positive HMCC responded to inhibition of hGH by a hGH antagonist with a decrease in cell number, whereas hGH-negative HMCC did not. Conclusion: Primary HMCC proliferate in response to hGH, and the proliferation of hGH-positive HMCC is inhibited by hGH antagonism. Inhibition of hGH in patients with mammary carcinoma may therefore limit tumor growth. Copyright © 2011 by The Endocrine Society.
Source Title: Journal of Clinical Endocrinology and Metabolism
ISSN: 0021972X
DOI: 10.1210/jc.2011-0473
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.


checked on Aug 12, 2022


checked on Sep 27, 2021

Page view(s)

checked on Aug 18, 2022

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.