Please use this identifier to cite or link to this item:
|Title:||A new strategy to induce effective antitumour response in vitro and in vivo||Authors:||Wang, M.
|Issue Date:||Sep-2008||Citation:||Wang, M., Xie, Z., Shi, M., Lu, H., Yu, M., Hu, M., Lu, F., Ma, Y., Shen, B., Guo, N. (2008-09). A new strategy to induce effective antitumour response in vitro and in vivo. Scandinavian Journal of Immunology 68 (3) : 287-296. ScholarBank@NUS Repository. https://doi.org/10.1111/j.1365-3083.2008.02140.x||Abstract:||To induce Her2-specific cell immune response, we used xenogeneic antigen rat neu L2-S2 domains as the vaccine antigen. The antigenic protein was engineered as a chimeric protein with human IgG1 Fc region (neu-Fc). Neu-Fc could stimulate the cell proliferation in mixed lymphocyte reaction effectively. Simultaneous neu-Fc and IFN-γ stimulation dramatically elevated IL-12 secretion and reduced IL-10 production in PBMC. To further augment the activating effects on Th1-type response, Bacille Calmette-Guerin (BCG) was utilized as a non-specific stimulus. Neu-Fc, IFN-γ and BCG costimulation exhibited the most conspicuous effects on the reversal of the Th1-type inhibitory effects by MCF-7 cell supernatant compared with neu-Fc alone or IFN-γ and BCG costimulation. The lytic activity of effector cells to Her2 overexpressing cells was greatly promoted by neu-Fc, IFN-γ and BCG stimulation simultaneously. Neu-Fc led to considerable retardation in EMT6/Her2 tumour growth in Balb/c mice. IFN-γ and BCG efficiently enhanced the antitumour activity. A large amount of inflammatory cells were found to be accumulated in the tumour tissues or surrounded tumours in mice treated with neu-Fc, IFN-γ and BCG but no inflammatory cell infiltration was observed in control tumours, indicating that the strategy is potent enough to support the initiation and propagation of tumour-specific immune response in an established tumour and generate a proinflammatory environment. © 2008 The Authors.||Source Title:||Scandinavian Journal of Immunology||URI:||http://scholarbank.nus.edu.sg/handle/10635/109146||ISSN:||03009475||DOI:||10.1111/j.1365-3083.2008.02140.x|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Mar 27, 2020
WEB OF SCIENCETM
checked on Mar 19, 2020
checked on Mar 28, 2020
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.