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Title: Identification of a subpopulation of nasopharyngeal carcinoma cells with cancer stem-like cell properties by high aldehyde dehydrogenase activity
Authors: Yu, F.
Sim, A.C.N.
Li, C. 
Li, Y.
Zhao, X.
Wang, D.-Y.
Loh, K.S.
Keywords: aldehyde dehydrogenase
C666-1 cell line
cancer stem-like cells
Epstein-Barr virus associated
Nasopharyngeal carcinoma
therapy resistant
Issue Date: Aug-2013
Citation: Yu, F., Sim, A.C.N., Li, C., Li, Y., Zhao, X., Wang, D.-Y., Loh, K.S. (2013-08). Identification of a subpopulation of nasopharyngeal carcinoma cells with cancer stem-like cell properties by high aldehyde dehydrogenase activity. Laryngoscope 123 (8) : 1903-1911. ScholarBank@NUS Repository.
Abstract: Objectives/Hypothesis Cancer stem cells have been reported as a new therapeutic target in many cancers, but their existence in nasopharyngeal carcinoma (NPC) is largely unknown. This study was conducted to determine cancer stem-like cells in NPC cell line. Study Design Basic science experimental study. Methods Aldehyde dehydrogenase (ALDH) activity, a putative functional marker for cancer stem cells, was assessed in Epstein-Barr virus-associated NPC cell line C666-1 cells. The ability of cells with high and low ALDH activity to proliferate, resist therapy, and initiate tumor formation was compared. Results Enrichment of cancer stem-like cells (with high ALDH activity) in C666-1 was associated with a significantly greater ability to proliferate, be clonogenic, resist chemotherapy drugs and radiation, reconstitute a heterogeneous population, and express pluripotent markers. Furthermore, subcutaneous injection of these cells into immunodeficient nude mice resulted in a tendency of tumor formation at a higher rate as compared to cells with low ALDH activity. Conclusions These results provide evidence for the existence of cancer stem-like cells in the NPC cell line C666-1 cells. Laryngoscope, 123:1903-1911, 2013 © 2013 The American Laryngological, Rhinological and Otological Society, Inc.
Source Title: Laryngoscope
ISSN: 0023852X
DOI: 10.1002/lary.24003
Appears in Collections:Staff Publications

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