Please use this identifier to cite or link to this item:
|Title:||Cerebrospinal fluid of postherpetic neuralgia patients induced interleukin-6 release in human glial cell-line T98G||Authors:||Tay, A.S.
|Issue Date:||2013||Citation:||Tay, A.S., Liu, E.H., Lee, T.L., Miyazaki, S., Nishimura, W., Minami, T., Chan, Y.H., Low, C.-M., Tachibana, S. (2013). Cerebrospinal fluid of postherpetic neuralgia patients induced interleukin-6 release in human glial cell-line T98G. Neurochemistry International 63 (5) : 517-521. ScholarBank@NUS Repository. https://doi.org/10.1016/j.neuint.2013.08.007||Abstract:||Chronic intractable pain caused by postherpetic neuralgia (PHN) can be alleviated by intrathecal (i.t.) steroid therapy. We investigated the possibility that interleukin-6 (IL-6) release in an in vitro system could be a potential marker for evaluating the effectiveness of i.t. steroid therapy in PHN patients. We studied 32 patients who received a course of i.t. injection of water-soluble dexamethasone. Their therapeutic index was calculated as such: ((Pain score before treatment - Pain score after treatment) ÷ Pain score before treatment) × 100%, and they were divided into two groups, therapy effective (index > 50%) and ineffective (index < 50%). Cerebrospinal fluid (CSF) from the patients was used to stimulate cultures of T98G glioblastoma cells, and the subsequent IL-6 release was measured by enzyme-linked immunosorbent assay (ELISA). Our results showed that the CSF triggered IL-6 release from T98G cells in a volume-dependent manner. IL-6 release was significantly lower when using CSF from the therapy effective patient group (p < 0.001) compared to the therapy ineffective group. In particular, therapy effective patients had less IL-6 release even before treatment as compared to therapy ineffective patients. In the therapy effective group, in vitro steroid treatment suppressed the CSF's IL-6 releasing effect almost completely, whereas in the therapy ineffective group, the IL-6 release was significantly reduced but remained detectable. These in vitro tests may provide an objective evaluation on the efficacy of i.t. steroid therapy administered to PHN patients. © 2013 Elsevier Ltd. All rights reserved.||Source Title:||Neurochemistry International||URI:||http://scholarbank.nus.edu.sg/handle/10635/108291||ISSN:||01970186||DOI:||10.1016/j.neuint.2013.08.007|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Sep 19, 2020
WEB OF SCIENCETM
checked on Sep 11, 2020
checked on Sep 19, 2020
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.