Please use this identifier to cite or link to this item: https://doi.org/10.1007/s10495-013-0851-5
Title: An anthraquinone derivative, emodin sensitizes hepatocellular carcinoma cells to TRAIL induced apoptosis through the induction of death receptors and downregulation of cell survival proteins
Authors: Subramaniam, A.
Loo, S.Y.
Rajendran, P.
Manu, K.A. 
Perumal, E.
Li, F.
Shanmugam, M.K. 
Siveen, K.S.
Park, J.-I.
Ahn, K.S.
Hui, K.M.
Kumar, A.P. 
Sethi, G.
Keywords: Apoptosis
CHOP
DR4
DR5
Emodin
HCC
TRAIL
Issue Date: 2013
Citation: Subramaniam, A., Loo, S.Y., Rajendran, P., Manu, K.A., Perumal, E., Li, F., Shanmugam, M.K., Siveen, K.S., Park, J.-I., Ahn, K.S., Hui, K.M., Kumar, A.P., Sethi, G. (2013). An anthraquinone derivative, emodin sensitizes hepatocellular carcinoma cells to TRAIL induced apoptosis through the induction of death receptors and downregulation of cell survival proteins. Apoptosis 18 (10) : 1175-1187. ScholarBank@NUS Repository. https://doi.org/10.1007/s10495-013-0851-5
Abstract: Recombinant tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is currently under clinical trials for cancer, however many tumor cells, including hepatocellular carcinoma (HCC) develop resistance to TRAIL-induced apoptosis. Hence, novel agents that can alleviate TRAIL-induced resistance are urgently needed. In the present report, we investigated the potential of emodin to enhance apoptosis induced by TRAIL in HCC cells. As observed by MTT cytotoxicity assay and the externalization of the membrane phospholipid phosphatidylserine, we found that emodin can significantly potentiate TRAIL-induced apoptosis in HCC cells. When investigated for the mechanism(s), we observed that emodin can downregulate the expression of various cell survival proteins, and induce the cell surface expression of both TRAIL receptors, death receptors (DR) 4 as well as 5. In addition, emodin increased the expression of C/EBP homologous protein (CHOP) in a time-dependent manner. Knockdown of CHOP by siRNA decreased the induction of emodin-induced DR5 expression and apoptosis. Emodin-induced induction of DR5 was mediated through the generation of reactive oxygen species (ROS), as N-acetylcysteine blocked the induction of DR5 and the induction of apoptosis. Also, the knockdown of X-linked inhibitor of apoptosis protein by siRNA significantly reduced the sensitization effect of emodin on TRAIL-induced apoptosis. Overall, our experimental results clearly indicate that emodin can indeed potentiate TRAIL-induced apoptosis through the downregulation of antiapoptotic proteins, increased expression of apoptotic proteins, and ROS mediated upregulation of DR in HCC cells. © 2013 Springer Science+Business Media New York.
Source Title: Apoptosis
URI: http://scholarbank.nus.edu.sg/handle/10635/108255
ISSN: 13608185
DOI: 10.1007/s10495-013-0851-5
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