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Title: Bone marrow-derived cells that home to acoustic deafened cochlea preserved their hematopoietic identity
Authors: Tan, B.T.G.
Lee, M.M.G.
Ruan, R. 
Keywords: Bone marrow transplantation
Hematopoietic stem cell
Inner ear repair
Noise-induced hearing loss
Issue Date: 10-Jul-2008
Citation: Tan, B.T.G., Lee, M.M.G., Ruan, R. (2008-07-10). Bone marrow-derived cells that home to acoustic deafened cochlea preserved their hematopoietic identity. Journal of Comparative Neurology 509 (2) : 167-179. ScholarBank@NUS Repository.
Abstract: The high degree of bone marrow cell (BMC) plasticity has prompted us to test its restoration possibility in inner ear repair. Our aim was to determine the potential of these cells to transdifferentiate into specialized cochlea cell types after acoustic injury and BMC mobilization. Lethally irradiated mice were transplanted with BMCs from green fluorescent protein (GFP) transgenic mice and subjected to acoustic deafening 3 months later. In a separate experiment, stem cell factor and granulocyte colony-stimulating factor were administered to test the effect of BMC mobilization on bone marrow-derived cell (BMDC) transdifferentiation. All mice showed almost complete chimerism 3 months after bone marrow transplantation. Upon acoustic trauma, robust BMDC migration was observed in the deafened cochlea. GFP+ cell migration was most prominent during the first week after acoustic deafening, and these cells accumulated significantly at the spiral ligament, perilymphatic compartment walls, and limbus regions. Most of the BMDCs expressed CD45 and CD68 and were identified as macrophages. Upregulation of stromal-derived factor 1 (SDF-1) was also observed in the spiral ligament during the first week after acoustic deafening. Cytokine treatment resulted in increased BMC mobilization in the systemic circulation. However, the presence of any stem cell progenitors or the differentiation of BMDCs into any cell types expressing cochlea sensory, supporting, fibrocytic, or neuronal markers were not detected in the deafened cochlea. In conclusion, we have demonstrated the homing capability of BMDCs to the deafened cochlea, and these cells displayed mature hematopoietic properties without spontaneous transdifferentiation to any cochlea cell types after acoustic trauma or bone marrow mobilization. © 2008 Wiley-Liss, Inc.
Source Title: Journal of Comparative Neurology
ISSN: 00219967
DOI: 10.1002/cne.21729
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