Please use this identifier to cite or link to this item: https://doi.org/10.1006/abbi.1993.1553
DC FieldValue
dc.titleIsolation and characterization of pectin methylesterase from papaya
dc.contributor.authorLim, Y.-M.
dc.contributor.authorChung, M.C.M.
dc.date.accessioned2014-11-10T09:52:12Z
dc.date.available2014-11-10T09:52:12Z
dc.date.issued1993
dc.identifier.citationLim, Y.-M., Chung, M.C.M. (1993). Isolation and characterization of pectin methylesterase from papaya. Archives of Biochemistry and Biophysics 307 (1) : 15-20. ScholarBank@NUS Repository. https://doi.org/10.1006/abbi.1993.1553
dc.identifier.issn00039861
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/107759
dc.description.abstractPectin methylesterase (PME) (EC 3.1.1.11) has been purified to apparent homogeneity from ripe papaya fruits. The purification protocol consisted of ammonium sulphate precipitation (60-80%) and cation exchange chromatography in CM Sepharose CL-6B and Mono S. Papaya PME consists of two components (PME 1 and PME 2), which have been shown to be isoenzymes by Ferguson plot analysis. The molecular weight of the enzyme is 27,000 while its isoelectric point is greater than pH 9.0. The N-terminal sequences of PME 1 and PME 2 are SVVTPNAVVADDGVFXFKTG. Both PME 1 and PME 2 showed optimum activities at pH 8.0 and 35°C. The average K(m)s of PME 1 and PME 2 are 0.0071 and 0.0166 g/liter pectin respectively, while the corresponding average V(max)s are 741 and 800 μmol methanol/min/mg protein, respectively. Papaya pectin methylesterase is activated by cations, but the effect is more pronounced for divalent than monovalent cations. Inhibition studies showed that sucrose is a noncompetitive inhibitor while p-chloromercuribenzoic acid has no significant effect on its activity.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1006/abbi.1993.1553
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentBIOCHEMISTRY
dc.description.doi10.1006/abbi.1993.1553
dc.description.sourcetitleArchives of Biochemistry and Biophysics
dc.description.volume307
dc.description.issue1
dc.description.page15-20
dc.description.codenABBIA
dc.identifier.isiutA1993MG68300003
Appears in Collections:Staff Publications

Show simple item record
Files in This Item:
There are no files associated with this item.

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.