Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.joen.2010.02.022
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dc.titleImmunogenic Potential of Enterococcus faecalis Biofilm under Simulated Growth Conditions
dc.contributor.authorMathew, S.
dc.contributor.authorYaw-Chyn, L.
dc.contributor.authorKishen, A.
dc.date.accessioned2014-11-06T08:24:07Z
dc.date.available2014-11-06T08:24:07Z
dc.date.issued2010-05
dc.identifier.citationMathew, S., Yaw-Chyn, L., Kishen, A. (2010-05). Immunogenic Potential of Enterococcus faecalis Biofilm under Simulated Growth Conditions. Journal of Endodontics 36 (5) : 832-836. ScholarBank@NUS Repository. https://doi.org/10.1016/j.joen.2010.02.022
dc.identifier.issn00992399
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/107466
dc.description.abstractIntroduction: The aim of this study was to evaluate the effect of growth modes (planktonic and biofilms) of Enterococcus faecalis on the intracellular survival and ability to produce tumor necrosis factor α (TNF-α) and interleukin (IL)-6 after interacting with monocytes/in vitro-differentiated macrophages. Methods: In vitro biofilms of three E. faecalis strains (ATCC-29212, OG1RF, and FA2-2) were grown on dentin under simulated nutrient-rich and nutrient-deprived conditions. Biofilm-derived E. faecalis cells were incubated with monocytes/in vitro-differentiated human macrophages. A fluorometric assay was used to quantify the surface adherent bacteria, whereas an antibiotic protection assay was used to quantify the internalized E. faecalis cells 6 to 48 hours after interaction with macrophages. TNF-α and IL-6 produced during this interaction were quantified by an enzyme-linked immunosorbent assay. Results: The surface adherence and intracellular survival of E. faecalis within macrophages was significantly higher in biofilm bacteria when compared with planktonic cells (p ≤ 0.05). E. faecalis strains survived within the macrophages for up to 48 hours and produced higher levels of IL-6 with planktonic cells when compared with biofilm cells. Except in FA2-2, the planktonic cells of other strains did not show a significant increase in TNF-α compared with the biofilm cells (p > 0.05). Conclusion: Biofilm cells of E. faecalis interacting with macrophages showed higher potential for surface adherence, intracellular survival and produced lesser amounts of IL-6 and TNF-α when compared to planktonic cells. Further experiments are required to understand the clinical implication of the intracellular survival of E. faecalis biofilm. © 2010 American Association of Endodontists.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.joen.2010.02.022
dc.sourceScopus
dc.subjectBacterial biofilm
dc.subjectEnterococcus facealis
dc.subjectimmunogenic potential
dc.subjectmacrophages
dc.subjectnutrient deprivation
dc.typeArticle
dc.contributor.departmentPATHOLOGY
dc.description.doi10.1016/j.joen.2010.02.022
dc.description.sourcetitleJournal of Endodontics
dc.description.volume36
dc.description.issue5
dc.description.page832-836
dc.description.codenJOEND
dc.identifier.isiut000277768900011
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