Techniques in the culturing and handling of Liposcelis entomophilus (Enderlein) (Psocoptera: Liposcelidae)

Abstract

Liposcelis entomophilus (Enderlein) was maintained on diets composed of various combinations of wheat flour, skimmed milk powder and yeast. After 6 weeks, a diet comprising the above components in 10:10:1 proportions respectively resulted in the highest increase in liposcelid population (P < 0.05). Subsequently, L. entomophilus was successfully cultured on this medium at 30 ± 1°C and 72-78% r.h. A method for maintaining stock cultures was established. The use of an aspirator in the handling of these liposcelids was tested and proved adequate, the optimum suction pressure being 60 mmHg (P < 0.05). A separating sieve, used with a 60 W incandescent bulb, was specially constructed to extract these liposcelids from infested grains. Modifications to this apparatus and its other uses are discussed. © 1990.