Please use this identifier to cite or link to this item:
Title: Tannic acid, a potent inhibitor of epidermal growth factor receptor tyrosine kinase
Authors: Yang, E.B.
Wei, L.
Zhang, K.
Chen, Y.Z. 
Chen, W.N.
Keywords: EGF receptor
Molecular modeling
Tyrosine kinase
Issue Date: Mar-2006
Citation: Yang, E.B., Wei, L., Zhang, K., Chen, Y.Z., Chen, W.N. (2006-03). Tannic acid, a potent inhibitor of epidermal growth factor receptor tyrosine kinase. Journal of Biochemistry 139 (3) : 495-502. ScholarBank@NUS Repository.
Abstract: Increasing evidence supports the hypothesis that tannic acid, a plant polyphenol, exerts anticarcinogenic activity in chemically induced cancers. In the present study, tannic acid was found to strongly inhibit tyrosine kinase activity of epidermal growth factor receptor (EGFr) in vitro (IC50 = 323 nM). In contrast, the inhibition by tannic acid of p60c-src tyrosine kinase (IC50 = 14 μM) and insulin receptor tyrosine kinase (IC50 = 5 μM) was much weaker. The inhibition of EGFr tyrosine kinase by tannic acid was competitive with respect to ATP and non-competitive with respect to peptide substrate. In cultured cells, growth factor-induced tyrosine phosphorylation of growth factor receptors, including EGFr, platelet-derived growth factor receptor, and basic fibroblast growth factor receptor, was inhibited by tannic acid. No inhibition of insulin-induced tyrosine phosphorylation of insulin receptor and insulin-receptor substrate-1 was observed. EGF-stimulated growth of HepG2 cells was inhibited in the presence of tannic acid. The inhibition of serine/threonine-specific protein kinases, including cAMP-dependent protein kinase, protein kinase C and mitogen-activated protein kinase, by tannic acid was only detected at relatively high concentration, IC50 being 3, 325 and 142 μM respectively. The molecular modeling study suggested that tannic acid could be docked into the ATP binding pockets of either EGFr or insulin receptor. These results demonstrate that tannic acid is an in vitro potent inhibitor of EGFr tyrosine kinase. © 2006 The Japanese Biochemical Society.
Source Title: Journal of Biochemistry
ISSN: 0021924X
DOI: 10.1093/jb/mvj050
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.


checked on Feb 19, 2020


checked on Feb 19, 2020

Page view(s)

checked on Feb 15, 2020

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.