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|Title:||Quantification of oxyresveratrol analog trans-2,4,3',5'-tetramethoxystilbene in rat plasma by a rapid HPLC method: Application in a pre-clinical pharmacokinetic study||Authors:||Lin, H.-S.
|Keywords:||Absolute oral bioavailability
|Issue Date:||Dec-2010||Citation:||Lin, H.-S., Choo, Q.-Y., Ho, P.C. (2010-12). Quantification of oxyresveratrol analog trans-2,4,3',5'-tetramethoxystilbene in rat plasma by a rapid HPLC method: Application in a pre-clinical pharmacokinetic study. Biomedical Chromatography 24 (12) : 1373-1378. ScholarBank@NUS Repository. https://doi.org/10.1002/bmc.1454||Abstract:||A rapid HPLC method was developed and validated for the quantification of oxyresveratrol analog trans-2,4,3',5'-tetramethoxystilbene (oxyresveratrol tetramethyl ether, OTE) in rat plasma. Chromatographic separation was achieved on an RP-HPLC column, which was protected by a guard column through a 12 min gradient delivery of a mixture of acetonitrile-water at 50°C. The UV absorbance at 325 nm was recorded. The retention time of OTE and trans-stilbene (internal standard) was about 7.7 and 8.4 min, respectively. The calibration curves were linear (R2 ≥ 0.9986) with a lower limit of quantification of 15 ng/mL. The intra- and inter-day variations, in terms of RSD, were all lower than 9.8% while the intra-day and inter-day bias ranged from -8.3 to +9.2%. The pharmacokinetics of OTE was assessed in rats using 2-hydroxypropyl-β-cyclodextrin as a dosing vehicle. After intravenous administration, OTE possessed a long terminal elimination half-life (t1/2 λz = 481 ± 137 min) and slow clearance (Cl = 29.1 ± 3.7 mL/min/kg). Upon oral administration, OTE was rapidly absorbed. However, it only displayed minimal plasma exposure and its absolute oral bioavailability (F) was as low as 4.5 ± 3.2%. Fortunately, the levels of OTE after single oral administration were sufficient to inhibit human cytochrome P450 1B1. © 2010 John Wiley & Sons, Ltd.||Source Title:||Biomedical Chromatography||URI:||http://scholarbank.nus.edu.sg/handle/10635/106275||ISSN:||02693879||DOI:||10.1002/bmc.1454|
|Appears in Collections:||Staff Publications|
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