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https://doi.org/10.1016/j.jpba.2008.10.042
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dc.title | A rapid HPLC method for the quantification of 3,5,4′-trimethoxy-trans-stilbene (TMS) in rat plasma and its application in pharmacokinetic study | |
dc.contributor.author | Lin, H.-S. | |
dc.contributor.author | Ho, P.C. | |
dc.date.accessioned | 2014-10-29T01:47:48Z | |
dc.date.available | 2014-10-29T01:47:48Z | |
dc.date.issued | 2009-02-20 | |
dc.identifier.citation | Lin, H.-S., Ho, P.C. (2009-02-20). A rapid HPLC method for the quantification of 3,5,4′-trimethoxy-trans-stilbene (TMS) in rat plasma and its application in pharmacokinetic study. Journal of Pharmaceutical and Biomedical Analysis 49 (2) : 387-392. ScholarBank@NUS Repository. https://doi.org/10.1016/j.jpba.2008.10.042 | |
dc.identifier.issn | 07317085 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/105589 | |
dc.description.abstract | A rapid HPLC-UV method had been developed and validated to quantify 3,5,4′-trimethoxy-trans-stilbene (TMS), a naturally occurring and pharmacologically active analog of resveratrol in rat plasma. The samples were mixed with three volumes of acetonitrile to precipitate protein. Chromatographic separation was achieved on a RP-HPLC column (Agilent ZORBAX Eclipse Plus C18: 250 mm × 4.6 mm i.d., 5 μm), which was protected by a guard column (Agilent ZORBAX Eclipse Plus C18: 12.5 mm × 4.6 mm i.d., 5 μm) through isocratic delivery of a mobile phase of acetonitrile: water (75:25, v/v) at a flow rate of 1.2 ml/min. The assay was executed at 30 °C and the UV absorbance at 320 nm was monitored. The retention time of TMS and trans-stilbene (internal standard) was 6.5 and 8.3 min, respectively. The calibration curve was linear within the range of 15-1000 ng/ml (R2 > 0.998) and 15 ng/ml was the lower LOQ. The intra- and inter-day precisions were good and the RSD was all lower than 7.3%. The mean absolute recovery of TMS in plasma ranged from 99.2 to 104.1%. This HPLC method had been successfully applied to study the pharmacokinetics of TMS, which was fully dissolved with hydroxypropyl-β-cyclodextrin (HP-β-CyD). In comparison with resveratrol, TMS had greater plasma exposure, longer elimination half-life and lower clearance. As TMS had superior pharmacokinetic characteristics, its potential as a preventive or therapeutic agent in resveratrol-effective conditions or diseases should be considered. © 2008 Elsevier B.V. All rights reserved. | |
dc.description.uri | http://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.jpba.2008.10.042 | |
dc.source | Scopus | |
dc.subject | 3,5,4′-trimethoxy-trans-stilbene | |
dc.subject | HPLC | |
dc.subject | Pharmacokinetics | |
dc.subject | UV detection | |
dc.type | Article | |
dc.contributor.department | PHARMACY | |
dc.description.doi | 10.1016/j.jpba.2008.10.042 | |
dc.description.sourcetitle | Journal of Pharmaceutical and Biomedical Analysis | |
dc.description.volume | 49 | |
dc.description.issue | 2 | |
dc.description.page | 387-392 | |
dc.description.coden | JPBAD | |
dc.identifier.isiut | 000263434900029 | |
Appears in Collections: | Staff Publications |
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