Please use this identifier to cite or link to this item:
https://scholarbank.nus.edu.sg/handle/10635/105582
DC Field | Value | |
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dc.title | A peptide derived from LFA-1 protein that modulates T-cell adhesion binds to soluble ICAM-1 protein | |
dc.contributor.author | Jois, S.D.S. | |
dc.contributor.author | Siahaan, T.J. | |
dc.date.accessioned | 2014-10-29T01:47:43Z | |
dc.date.available | 2014-10-29T01:47:43Z | |
dc.date.issued | 2003-04 | |
dc.identifier.citation | Jois, S.D.S.,Siahaan, T.J. (2003-04). A peptide derived from LFA-1 protein that modulates T-cell adhesion binds to soluble ICAM-1 protein. Journal of Biomolecular Structure and Dynamics 20 (5) : 635-644. ScholarBank@NUS Repository. | |
dc.identifier.issn | 07391102 | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/105582 | |
dc.description.abstract | Leukocyte function associated antigen 1 (LFA-1) and intercellular adhesion molecule 1 (ICAM-1) have been shown to be critical for adhesion process and immune response. Modulation or inhibition of the interaction between LFA-1/ICAM-1 interactions can result in therapeutic effects. Our group and others have shown that peptides derived from ICAM-1 or LFA-1 inhibit adhesion in a homotypic T-cell adhesion assay. It is likely that the peptides derived from ICAM-1 bind to LFA-1 and peptides derived from LFA-1 bind to ICAM-1 and inhibit the adhesion interaction. However, there are no concrete experimental evidence to show that peptides bind to either LFA-1 or ICAM-1 and inhibit the adhesion. Using NMR, CD and docking studies we have shown that an LFA-1 derived peptide binds to soluble ICAM-1. Docking studies using "autodock" resulted in LFA-1 peptide interacting with the ICAM-1 protein near Glu34. The proposed model based on our experimental data indicated that the LFA-1 peptide interacts with the protein via three intermolecular hydrogen bonds. Hydrophobic interactions also play a role in stabilizing the complex. | |
dc.source | Scopus | |
dc.subject | Cell adhesion inhibition | |
dc.subject | Docking | |
dc.subject | ICAM-1/LFA-1 | |
dc.subject | Peptide-protein complex | |
dc.subject | TRNOESY | |
dc.type | Article | |
dc.contributor.department | PHARMACY | |
dc.description.sourcetitle | Journal of Biomolecular Structure and Dynamics | |
dc.description.volume | 20 | |
dc.description.issue | 5 | |
dc.description.page | 635-644 | |
dc.description.coden | JBSDD | |
dc.identifier.isiut | NOT_IN_WOS | |
Appears in Collections: | Staff Publications |
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