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Title: Statistical analysis of binary data generated from multilocus dominant DNA markers
Authors: Khang, T.F.
Yap, V.B. 
Keywords: Ascertainment bias
Binary data
Dominant markers
Empirical Bayes
Root mean square error
Issue Date: Nov-2010
Citation: Khang, T.F., Yap, V.B. (2010-11). Statistical analysis of binary data generated from multilocus dominant DNA markers. Molecular Ecology Resources 10 (6) : 995-1008. ScholarBank@NUS Repository.
Abstract: The use of methodologies such as RAPD and AFLP for studying genetic variation in natural populations is widespread in the ecology community. Because data generated using these methods exhibit dominance, their statistical treatment is less straightforward. Several estimators have been proposed for estimating population genetic parameters, assuming simple random sampling and the Hardy-Weinberg (HW) law. The merits of these estimators remain unclear because no comparative studies of their theoretical properties have been carried out. Furthermore, ascertainment bias has not been explicitly modelled. Here, we present a comparison of a set of candidate estimators of null allele frequency (q), locus-specific heterozygosity (h) and average heterozygosity in terms of their bias, standard error, and root mean square error (RMSE). For estimating q and h, we show that none of the estimators considered has the least RMSE over the parameter space. Our proposed zero-correction procedure, however, generally leads to estimators with improved RMSE. Assuming a beta model for the distribution of null homozygote proportions, we show how correction for ascertainment bias can be carried out using a linear transform of the sample average of h and the truncated beta-binomial likelihood. Simulation results indicate that the maximum likelihood and empirical Bayes estimator of have negligible bias and similar RMSE. Ascertainment bias in estimators of is most pronounced when the beta distribution is J-shaped and negligible when the latter is inverse J-shaped. The validity of the current findings depends importantly on the HW assumption-a point that we illustrate using data from two published studies. © 2010 Blackwell Publishing Ltd.
Source Title: Molecular Ecology Resources
ISSN: 1755098X
DOI: 10.1111/j.1755-0998.2010.02845.x
Appears in Collections:Staff Publications

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