Please use this identifier to cite or link to this item: https://doi.org/10.1186/1471-2199-11-24
DC FieldValue
dc.titleTranscriptomic analysis of the venom gland of the red-headed krait (Bungarus flaviceps) using expressed sequence tags
dc.contributor.authorSiang, A.S.
dc.contributor.authorDoley, R.
dc.contributor.authorVonk, F.J.
dc.contributor.authorKini, R.M.
dc.date.accessioned2014-10-27T08:44:09Z
dc.date.available2014-10-27T08:44:09Z
dc.date.issued2010-03-29
dc.identifier.citationSiang, A.S., Doley, R., Vonk, F.J., Kini, R.M. (2010-03-29). Transcriptomic analysis of the venom gland of the red-headed krait (Bungarus flaviceps) using expressed sequence tags. BMC Molecular Biology 11 : -. ScholarBank@NUS Repository. https://doi.org/10.1186/1471-2199-11-24
dc.identifier.issn14712199
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/102063
dc.description.abstractBackground: The Red-headed krait (Bungarus flaviceps, Squamata: Serpentes: Elapidae) is a medically important venomous snake that inhabits South-East Asia. Although the venoms of most species of the snake genus Bungarus have been well characterized, a detailed compositional analysis of B. flaviceps is currently lacking.Results: Here, we have sequenced 845 expressed sequence tags (ESTs) from the venom gland of a B. flaviceps. Of the transcripts, 74.8% were putative toxins; 20.6% were cellular; and 4.6% were unknown. The main venom protein families identified were three-finger toxins (3FTxs), Kunitz-type serine protease inhibitors (including chain B of β-bungarotoxin), phospholipase A2 (including chain A of β-bungarotoxin), natriuretic peptide (NP), CRISPs, and C-type lectin.Conclusion: The 3FTxs were found to be the major component of the venom (39%). We found eight groups of unique 3FTxs and most of them were different from the well-characterized 3FTxs. We found three groups of Kunitz-type serine protease inhibitors (SPIs); one group was comparable to the classical SPIs and the other two groups to chain B of β-bungarotoxins (with or without the extra cysteine) based on sequence identity. The latter group may be functional equivalents of dendrotoxins in Bungarus venoms. The natriuretic peptide (NP) found is the first NP for any Asian elapid, and distantly related to Australian elapid NPs. Our study identifies several unique toxins in B. flaviceps venom, which may help in understanding the evolution of venom toxins and the pathophysiological symptoms induced after envenomation. © 2010 Siang et al; licensee BioMed Central Ltd.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1186/1471-2199-11-24
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1186/1471-2199-11-24
dc.description.sourcetitleBMC Molecular Biology
dc.description.volume11
dc.description.page-
dc.description.codenBMBMC
dc.identifier.isiut000277289100001
Appears in Collections:Staff Publications
Elements

Show simple item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
2010-transcriptomic_analysis_venom_gland-published.pdf1.19 MBAdobe PDF

OPEN

PublishedView/Download

SCOPUSTM   
Citations

31
checked on May 19, 2022

WEB OF SCIENCETM
Citations

33
checked on May 19, 2022

Page view(s)

384
checked on May 12, 2022

Download(s)

4
checked on May 12, 2022

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.