Please use this identifier to cite or link to this item: https://doi.org/10.1002/cyto.a.21164
Title: Three-dimensional segmentation of nuclei and mitotic chromosomes for the study of cell divisions in live Drosophila embryos
Authors: Chinta, R.
Wasser, M. 
Keywords: 3D nuclear segmentation
Cell cycle
Confocal microscopy
Drosophila
Embryogenesis
Histone-GFP
Live cell imaging
Mitosis
Issue Date: Jan-2012
Citation: Chinta, R., Wasser, M. (2012-01). Three-dimensional segmentation of nuclei and mitotic chromosomes for the study of cell divisions in live Drosophila embryos. Cytometry Part A 81 A (1) : 52-64. ScholarBank@NUS Repository. https://doi.org/10.1002/cyto.a.21164
Abstract: Drosophila embryogenesis is an established model to investigate mechanisms and genes related to cell divisions in an intact multicellular organism. Progression through the cell cycle phases can be monitored in vivo using fluorescently labeled fusion proteins andtime-lapse microscopy. To measure cellular properties in microscopic images, accurate and fast image segmentation methods are a critical prerequisite. To quantify static and dynamic features of interphase nuclei and mitotic chromosomes, we developed a three-dimensional (3D) segmentation method based on multiple level sets. We tested our method on 3D time-series images of live embryos expressing histone-2Av-green fluorescence protein. Our method is robust to low signal-to-noise ratios inherent to high-speed imaging, fluorescent signals in the cytoplasm, and dynamic changes of shape and texture. Comparisons with manual ground-truth segmentations showed that our method achieves more than 90% accuracy on the object as well as voxel levels and performs consistently throughout all cell cycle phases and developmental stages from syncytial blastoderm to postblastoderm mitotic domains. © 2011 International Society for Advancement of Cytometry.
Source Title: Cytometry Part A
URI: http://scholarbank.nus.edu.sg/handle/10635/102039
ISSN: 15524922
DOI: 10.1002/cyto.a.21164
Appears in Collections:Staff Publications

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