Please use this identifier to cite or link to this item: https://doi.org/10.1016/S0168-9452(98)00071-5
Title: The isolation, molecular characterization and expression of dihydroflavonol 4-reductase cDNA in the orchid, Bromheadia finlaysoniana
Authors: Liew, C.-F. 
Loh, C.-S. 
Goh, C.-J. 
Lim, S.-H. 
Keywords: Anthocyanin
Bromheadia finlaysoniana
CDNA
Dihydroflavonol 4- reductase
Orchid
Issue Date: 10-Jul-1998
Citation: Liew, C.-F., Loh, C.-S., Goh, C.-J., Lim, S.-H. (1998-07-10). The isolation, molecular characterization and expression of dihydroflavonol 4-reductase cDNA in the orchid, Bromheadia finlaysoniana. Plant Science 135 (2) : 161-169. ScholarBank@NUS Repository. https://doi.org/10.1016/S0168-9452(98)00071-5
Abstract: The cDNA, ODFR, encoding dihydroflavonol 4-reductase (DFR) was isolated from a cDNA library constructed from Bromheadia finlaysoniana flowers using a homologous probe generated via PCR. Southern blot analysis suggests that DFR is represented by a single gene in this orchid while Northern blot and reverse transcriptase-PCR analysis showed that DFR is expressed in all purple colored tissues. A phylogenetic tree constructed based on multiple alignments among DFR sequences showed that the DFR genes isolated from barley, maize and B. finlaysoniana, which are all monocotyledons, are more similar to each other than to DFR sequences from dicotyledonous plants.
Source Title: Plant Science
URI: http://scholarbank.nus.edu.sg/handle/10635/101955
ISSN: 01689452
DOI: 10.1016/S0168-9452(98)00071-5
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