Please use this identifier to cite or link to this item: https://doi.org/10.1160/TH04-03-0144
Title: The catalytic subunit of pseutarin C, a group C prothrombin activator from the venom of Pseudonaja textilis, is structurally similar to mammalian blood coagulation factor Xa
Authors: Rao, V.S.
Swarup, S. 
Kini, R.M. 
Keywords: Factor Xa
Procoagulants
Prothrombinase complex
Snake venom
Issue Date: Sep-2004
Citation: Rao, V.S., Swarup, S., Kini, R.M. (2004-09). The catalytic subunit of pseutarin C, a group C prothrombin activator from the venom of Pseudonaja textilis, is structurally similar to mammalian blood coagulation factor Xa. Thrombosis and Haemostasis 92 (3) : 509-521. ScholarBank@NUS Repository. https://doi.org/10.1160/TH04-03-0144
Abstract: Pseutarin C, a group C prothrombin activator from Pseudonaja textilis venom, is a large protein complex consisting of catalytic and nonenzymatic subunits, which are functionally similar to the mammalian FXa-FVa complex. Here, we present the complete cDNA sequence of the catalytic subunit of pseutarin C. The cDNA of the catalytic subunit encodes a protein of 449 amino acids, which includes a 22-residue signal peptide, 18-residue propeptide and a mature protein of 409 amino acids. The deduced amino acid sequence shows 74-83% identity to group D prothrombin activators from snake venom and ∼42% identity to mammalian FX and has identical domain structure. The precursor of the catalytic subunit of pseutarin C has several unique features. The activation peptide of the catalytic subunit of pseutarin C is significantly smaller (27 as compared to 52 residues in mammalian FX) and does not contain any glycosylation sites. Unlike coagulation FXa, Ser52 and Asn45 of the light and heavy chains are O- and N-glycosylated in pseutarin C catalytic subunit. There is a 12-residue insertion in pseutarin C catalytic subunit close to the region that is implicated in binding to FVa. This is the first sequence of the catalytic subunit of a group C prothrombin activator. © 2004 Schattauer GmbH, Stuttgart.
Source Title: Thrombosis and Haemostasis
URI: http://scholarbank.nus.edu.sg/handle/10635/101877
ISSN: 03406245
DOI: 10.1160/TH04-03-0144
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