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Title: Temporal and differential gene expression of Singapore grouper iridovirus
Authors: Chen, L.M.
Wang, F. 
Song, W.
Hew, C.L. 
Issue Date: Oct-2006
Citation: Chen, L.M., Wang, F., Song, W., Hew, C.L. (2006-10). Temporal and differential gene expression of Singapore grouper iridovirus. Journal of General Virology 87 (10) : 2907-2915. ScholarBank@NUS Repository.
Abstract: Singapore grouper iridovirus (SGIV), an iridovirus in the genus Ranavirus, is a major pathogen that results in significant economic losses in grouper aquaculture. To investigate further its infective mechanisms, for the first time, a viral DNA microarray was generated for the SGIV genome to measure the expression of its predicted open reading frames simultaneously in vitro. By using the viral DNA microarray, the temporal gene expression of SGIV was characterized and the DNA microarray data were consistent with the results of real-time RT-PCR studies. Furthermore, different-stage viral genes (i.e. immediate-early, early and late genes) of SGIV were uncovered by combining drug treatments and DNA microarray studies. These results should offer important insights into the replication and pathogenesis of iridoviruses. © 2006 SGM.
Source Title: Journal of General Virology
ISSN: 00221317
DOI: 10.1099/vir.0.82219-0
Appears in Collections:Staff Publications

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