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Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/101459
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dc.titlePrimer design and optimization for RAPD analysis of Nepenthes
dc.contributor.authorLim, S.H.
dc.contributor.authorPhua, D.C.Y.
dc.contributor.authorTan, H.T.W.
dc.date.accessioned2014-10-27T08:37:25Z
dc.date.available2014-10-27T08:37:25Z
dc.date.issued2000
dc.identifier.citationLim, S.H.,Phua, D.C.Y.,Tan, H.T.W. (2000). Primer design and optimization for RAPD analysis of Nepenthes. Biologia Plantarum 43 (1) : 153-155. ScholarBank@NUS Repository.
dc.identifier.issn00063134
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/101459
dc.description.abstractPrimers with higher G+C content produced better random amplified polymorphic DNA (RAPD) profiles in Nepenthes. The occurrence of clustered G's and C's in the center of the primer seemed also to influence the banding patterns. It was also observed that for certain polymerases, the use of different buffers other than that recommended by the manufacturer provided a better amplification profile for Nepenthes.
dc.sourceScopus
dc.subjectGC content
dc.subjectPCR buffer
dc.subjectRAPD markers
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.sourcetitleBiologia Plantarum
dc.description.volume43
dc.description.issue1
dc.description.page153-155
dc.description.codenBPABA
dc.identifier.isiutNOT_IN_WOS
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