Please use this identifier to cite or link to this item: https://doi.org/10.1099/vir.0.054460-0
DC FieldValue
dc.titleMedaka haploid embryonic stem cells are susceptible to Singapore grouper iridovirus as well as to other viruses of aquaculture fish species
dc.contributor.authorYuan, Y.
dc.contributor.authorHuang, X.
dc.contributor.authorZhang, L.
dc.contributor.authorZhu, Y.
dc.contributor.authorHuang, Y.
dc.contributor.authorQin, Q.
dc.contributor.authorHong, Y.
dc.date.accessioned2014-10-27T08:33:15Z
dc.date.available2014-10-27T08:33:15Z
dc.date.issued2013-10
dc.identifier.citationYuan, Y., Huang, X., Zhang, L., Zhu, Y., Huang, Y., Qin, Q., Hong, Y. (2013-10). Medaka haploid embryonic stem cells are susceptible to Singapore grouper iridovirus as well as to other viruses of aquaculture fish species. Journal of General Virology 94 (PART10) : 2352-2359. ScholarBank@NUS Repository. https://doi.org/10.1099/vir.0.054460-0
dc.identifier.issn00221317
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/101075
dc.description.abstractViral infection is a challenge in high-density aquaculture, as it leads to various diseases and causes massive or even complete loss. The identification and disruption of host factors that viruses utilize for infection offer a novel approach to generate viral-resistant seed stocks for costefficient and sustainable aquaculture. Genetic screening in haploid cell cultures represents an ideal tool for host factor identification. We have recently generated haploid embryonic stem (ES) cells in the laboratory fish medaka. Here, we report that HX1, one of the three established medaka haploid ES cell lines, was susceptible to the viruses tested and is thus suitable for genetic screening to identify host factors. HX1 cells displayed a cytopathic effect and massive death upon inoculation with three highly infectious and notifiable fish viruses, namely Singapore grouper iridovirus (SGIV), spring viremia of carp virus (SVCV) and red-spotted grouper nervous necrosis virus (RGNNV). Reverse transcription-PCR and Western blot analyses revealed the expression of virus genes. SGIV infection in HX1 cells elicited a host immune response and apoptosis. Viral replication kinetics were determined from a virus growth curve, and electron microscopy revealed propagation, assembly and release of infectious SGIV particles in HX1 cells. Our results demonstrate that medaka haploid ES cells are susceptible to SGIV, as well as to SVCV and RGNNV, offering a unique opportunity for the identification of host factors by genetic screening. © 2013 SGM.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1099/vir.0.054460-0
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1099/vir.0.054460-0
dc.description.sourcetitleJournal of General Virology
dc.description.volume94
dc.description.issuePART10
dc.description.page2352-2359
dc.description.codenJGVIA
dc.identifier.isiut000326215000021
Appears in Collections:Staff Publications

Show simple item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

18
checked on Nov 30, 2019

WEB OF SCIENCETM
Citations

18
checked on Jul 5, 2019

Page view(s)

30
checked on Nov 30, 2019

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.