Mapping protein-protein interfaces on the basis of proton density difference

Abstract

Making contact: A protein-protein interface (see structure) can be identified unambiguously from the dependence of amide-proton relaxation on proton density, as demonstrated by the identification of subunit contacts in hemoglobin. The information on upper limits for effective distances thus obtained allows the protein complex structure to be modeled more accurately with docking techniques. (Figure Presented) © 2005 Wiley-VCH Verlag GmbH & Co. KGaA.