Please use this identifier to cite or link to this item: https://doi.org/10.1146/annurev-physchem-040513-103641
Title: Imaging fluorescence fluctuation spectroscopy: New tools for quantitative bioimaging
Authors: Bag, N.
Wohland, T. 
Keywords: cumulant analysis
fluorescence correlation spectroscopy
fluorescence cross-correlation spectroscopy
moment analysis
quantitative bioimaging
Issue Date: 2014
Citation: Bag, N., Wohland, T. (2014). Imaging fluorescence fluctuation spectroscopy: New tools for quantitative bioimaging. Annual Review of Physical Chemistry 65 : 225-248. ScholarBank@NUS Repository. https://doi.org/10.1146/annurev-physchem-040513-103641
Abstract: Fluorescence fluctuation spectroscopy (FFS) techniques provide information at the single-molecule level with excellent time resolution. Usually applied at a single spot in a sample, they have been recently extended into imaging formats, referred to as imaging FFS. They provide spatial information at the optical diffraction limit and temporal information in the microsecond to millisecond range. This review provides an overview of the different modalities in which imaging FFS techniques have been implemented and discusses present imaging FFS capabilities and limitations. A combination of imaging FFS and nanoscopy would allow one to record information with the detailed spatial information of nanoscopy, which is ∼20 nm and limited only by fluorophore size and labeling density, and the time resolution of imaging FFS, limited by the fluorescence lifetime. This combination would provide new insights into biological events by providing spatiotemporal resolution at unprecedented levels. Copyright © 2014 by Annual Reviews.
Source Title: Annual Review of Physical Chemistry
URI: http://scholarbank.nus.edu.sg/handle/10635/100883
ISSN: 0066426X
DOI: 10.1146/annurev-physchem-040513-103641
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