Please use this identifier to cite or link to this item: https://doi.org/10.1021/pr1008916
DC FieldValue
dc.titleIdentification of novel proteins from the venom of a cryptic snake drysdalia coronoides by a combined transcriptomics and proteom cs approach
dc.contributor.authorChatrath, S.T.
dc.contributor.authorChapeaurouge, A.
dc.contributor.authorLin, Q.
dc.contributor.authorLim, T.K.
dc.contributor.authorDunstan, N.
dc.contributor.authorMirtschin, P.
dc.contributor.authorKumar, P.P.
dc.contributor.authorKini, R.M.
dc.date.accessioned2014-10-27T08:31:01Z
dc.date.available2014-10-27T08:31:01Z
dc.date.issued2011-02-04
dc.identifier.citationChatrath, S.T., Chapeaurouge, A., Lin, Q., Lim, T.K., Dunstan, N., Mirtschin, P., Kumar, P.P., Kini, R.M. (2011-02-04). Identification of novel proteins from the venom of a cryptic snake drysdalia coronoides by a combined transcriptomics and proteom cs approach. Journal of Proteome Research 10 (2) : 739-750. ScholarBank@NUS Repository. https://doi.org/10.1021/pr1008916
dc.identifier.issn15353893
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/100873
dc.description.abstractWe have investigated the transcriptome and proteome of the venom of a cryptic Australian elapid snake Drysdalia coronoides. To probe into the transcriptome, we constructed a partial cDNA library from the venom gland of D. coronoides. The proteome of the venom of D. coronoides was explored by tryptic digestion of the crude venom followed by HPLC separation of the resulting peptides and MALDI-TOF/TOF mass spectrometric analysis. Importantly, the tandem MS data of the tryptic peptides of the venom not only confirmed the predicted protein sequences deduced from the transcriptome, but also added to our knowledge about the venom composition through identification of two more toxin families. Using both the approaches, we were able to identify proteins belonging to eight different snake venom protein superfamilies, namely, three-finger toxins, serine protease inhibitors, cysteine rich secretory proteins, phospholipases A 2, venom nerve growth factors, snake venom metalloproteases, vespryns, and a new family phospholipase B. We also identified three novel proteins belonging to the three-finger toxin superfamily. © 2011 American Chemical Society.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1021/pr1008916
dc.sourceScopus
dc.subjectPhospholipases
dc.subjectProteome
dc.subjectTandem mass spectrometry
dc.subjectThree-finger toxins
dc.subjectTranscriptome
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1021/pr1008916
dc.description.sourcetitleJournal of Proteome Research
dc.description.volume10
dc.description.issue2
dc.description.page739-750
dc.description.codenJPROB
dc.identifier.isiut000286868500033
Appears in Collections:Staff Publications

Show simple item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

41
checked on May 20, 2022

WEB OF SCIENCETM
Citations

40
checked on May 20, 2022

Page view(s)

128
checked on May 12, 2022

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.