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Title: Host-specific encapsidation of a defective RNA 3 of Cucumber mosaic virus
Authors: Kaplan, I.B.
Lee, K.-C. 
Canto, T.
Wong, S.-M. 
Palukaitis, P.
Issue Date: Dec-2004
Citation: Kaplan, I.B., Lee, K.-C., Canto, T., Wong, S.-M., Palukaitis, P. (2004-12). Host-specific encapsidation of a defective RNA 3 of Cucumber mosaic virus. Journal of General Virology 85 (12) : 3757-3763. ScholarBank@NUS Repository.
Abstract: Defective (D) RNAs were generated in tobacco upon passage of two isolates of Cucumber mosaic virus (CMV) initially derived from RNA transcripts of cDNA clones. In both cases, the D RNA was derived by a single in-frame deletion of either 339 or 411 nt within the 3a gene of Fny-CMV RNA 3 or M-CMV RNA 3, respectively. The generation of D RNAs was rare and occurred with two CMV isolates, the virions of which were known to differ in physico-chemical properties. The Fny-CMV D RNA 3, designated D RNA 3-1, was maintained by passage together with Fny-CMV in tobacco, but was lost by passage in squash. D RNA 3-1 accumulated in the inoculated squash cotyledons but not in upper, systemically infected leaves. Virions purified from infected squash cotyledons or leaf mesophyll protoplasts did not contain D RNA 3-1. Therefore, the failure of D RNA 3-1 to accumulate in squash leaves systemically infected by CMV was due to a lack of encapsidation of the D RNA 3-1 and movement out of the inoculated leaves. © 2004 SGM.
Source Title: Journal of General Virology
ISSN: 00221317
DOI: 10.1099/vir.0.80345-0
Appears in Collections:Staff Publications

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