High frequency shoot regeneration from leaf disc explants of garland chrysanthemum (Chrysanthemum coronarium L.) in vitro

Abstract

An efficient tissue culture system for plant regeneration from cultured leaf discs of garland chrysanthemum (Chrysanthemum coronarium L.) was developed. Adventitious shoots were regenerated from explants, at a high frequency (73%), grown on Murashige and Skoog's (MS) medium containing benzyladenine (BA) and α-naphthaleneacetic acid (NAA), each at 2.5 μM. The shoot regenerability of the explant was further enhanced in the presence of 1 μM AgNO3. Explants grown in the presence of AgNO3 generally produced higher amounts of ethylene than control explants, although both explants showed a similar pattern of ethylene production. On the contrary, ethylene produced by explants grown in the presence of aminoethoxyvinylglycine remained low throughout the entire culture period of 35 days. More than 50% regenerated shoots were induced to form roots on MS medium supplemented with 5-25 μM indole-3-butyric acid.