Please use this identifier to cite or link to this item: https://doi.org/10.1016/S0168-9452(97)00098-8
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dc.titleHigh frequency shoot regeneration from leaf disc explants of garland chrysanthemum (Chrysanthemum coronarium L.) in vitro
dc.contributor.authorLee, T.
dc.contributor.authorHuang, M.E.E.
dc.contributor.authorPua, E.-C.
dc.date.accessioned2014-10-27T08:30:22Z
dc.date.available2014-10-27T08:30:22Z
dc.date.issued1997-08-08
dc.identifier.citationLee, T., Huang, M.E.E., Pua, E.-C. (1997-08-08). High frequency shoot regeneration from leaf disc explants of garland chrysanthemum (Chrysanthemum coronarium L.) in vitro. Plant Science 126 (2) : 219-226. ScholarBank@NUS Repository. https://doi.org/10.1016/S0168-9452(97)00098-8
dc.identifier.issn01689452
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/100826
dc.description.abstractAn efficient tissue culture system for plant regeneration from cultured leaf discs of garland chrysanthemum (Chrysanthemum coronarium L.) was developed. Adventitious shoots were regenerated from explants, at a high frequency (73%), grown on Murashige and Skoog's (MS) medium containing benzyladenine (BA) and α-naphthaleneacetic acid (NAA), each at 2.5 μM. The shoot regenerability of the explant was further enhanced in the presence of 1 μM AgNO3. Explants grown in the presence of AgNO3 generally produced higher amounts of ethylene than control explants, although both explants showed a similar pattern of ethylene production. On the contrary, ethylene produced by explants grown in the presence of aminoethoxyvinylglycine remained low throughout the entire culture period of 35 days. More than 50% regenerated shoots were induced to form roots on MS medium supplemented with 5-25 μM indole-3-butyric acid.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/S0168-9452(97)00098-8
dc.sourceScopus
dc.subjectAgNO3
dc.subjectChrysanthemum coronarium L.
dc.subjectGarland chrysanthemum
dc.subjectRooting
dc.subjectShoot regeneration
dc.subjectTissue culture
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1016/S0168-9452(97)00098-8
dc.description.sourcetitlePlant Science
dc.description.volume126
dc.description.issue2
dc.description.page219-226
dc.description.codenPLSCE
dc.identifier.isiutA1997XK97500010
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