Please use this identifier to cite or link to this item:
|Title:||Hibiscus chlorotic ringspot virus p27 and its isoforms affect symptom expression and potentiate virus movement in kenaf (Hibiscus cannabinus L.)||Authors:||Zhou, T.
|Keywords:||Noncanonical start codon
|Issue Date:||Sep-2006||Citation:||Zhou, T., Fan, Z.F., Li, H.F., Wong, S.M. (2006-09). Hibiscus chlorotic ringspot virus p27 and its isoforms affect symptom expression and potentiate virus movement in kenaf (Hibiscus cannabinus L.). Molecular Plant-Microbe Interactions 19 (9) : 948-957. ScholarBank@NUS Repository. https://doi.org/10.1094/MPMI-19-0948||Abstract:||Hibiscus chlorotic ringspot virus (HCRSV), a member of the genus Carmovirus, encodes p27 (27-kDa protein) and two other in-frame isoforms (p25 and p22.5) that are coterminal at the carboxyl end. Only p27, which initiates at the 2570CUG codon, was detected in transfected kenaf (Hibiscus cannabinus L.) protoplasts through fusion to a Flag tag at either its N or C terminus. Subcellular localization of a p27-green fluorescent fusion protein in kenaf epidermal cells showed that it was localized to membrane structures close to cell walls. To study the functions of these proteins, a number of start codon mutants and premature translation termination mutants were constructed. Phenotypic differences were observed between the wild-type virus and these mutants during infection. Infectivity assays on plants indicated that p27 is a determinant of symptom severity. Without p25, appearance of symptoms on systemically infected kenaf leaves was delayed by 4 to 8 days. In a timecourse analysis, Western blot assays revealed that the delay corresponded to retardation in virus systemic movement, which suggested that p25 is probably involved in virus systemic movement. Mutations disrupting expression of p22.5 did not affect symptoms or virus movement. © 2006 The American Phytopathological Society.||Source Title:||Molecular Plant-Microbe Interactions||URI:||http://scholarbank.nus.edu.sg/handle/10635/100821||ISSN:||08940282||DOI:||10.1094/MPMI-19-0948|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on May 7, 2021
WEB OF SCIENCETM
checked on May 7, 2021
checked on May 2, 2021
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.