Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.jbiosc.2012.10.004
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dc.titleHeart extracellular matrix supports cardiomyocyte differentiation of mouse embryonic stem cells
dc.contributor.authorHiguchi, S.
dc.contributor.authorLin, Q.
dc.contributor.authorWang, J.
dc.contributor.authorLim, T.K.
dc.contributor.authorJoshi, S.B.
dc.contributor.authorAnand, G.S.
dc.contributor.authorChung, M.C.M.
dc.contributor.authorSheetz, M.P.
dc.contributor.authorFujita, H.
dc.date.accessioned2014-10-27T08:29:54Z
dc.date.available2014-10-27T08:29:54Z
dc.date.issued2013-03
dc.identifier.citationHiguchi, S., Lin, Q., Wang, J., Lim, T.K., Joshi, S.B., Anand, G.S., Chung, M.C.M., Sheetz, M.P., Fujita, H. (2013-03). Heart extracellular matrix supports cardiomyocyte differentiation of mouse embryonic stem cells. Journal of Bioscience and Bioengineering 115 (3) : 320-325. ScholarBank@NUS Repository. https://doi.org/10.1016/j.jbiosc.2012.10.004
dc.identifier.issn13891723
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/100807
dc.description.abstractWe have evaluated the effect of heart extracellular matrix (ECM) on the cardiomyocyte differentiation of mouse embryonic stem cells (ES cells) using de-cellularized heart tissue. Several lines of evidence indicate that ECM plays significant roles in cell proliferation, cell death and differentiation, but role of ECM possessing a 3D structure in differentiation has not been studied in detail. We found that there are substantial differences in the quantitative protein profiles of ECM in SDS-treated heart tissue compared to that of liver tissue, as assessed by iTRAQ™ quantitative proteomics analysis. When mouse ES cells were cultured on thin (60 μm) sections of de-cellularized tissue, the expression of cardiac myosin heavy chain (cMHC) and cardiac troponin I (cTnI) was high in ES cells cultured on heart ECM compared with those cultured on liver ECM. In addition, the protein expression of cMHC and cTnI was detected in cells on heart ECM after 2 weeks, which was not detectable in cells on liver ECM. These results indicate that heart ECM plays a critical role in the cardiomyocyte differentiation of ES cells. We propose that tissue-specific ECM induced cell lineage specification through mechano-transduction mediated by the structure, elasticity and components of ECM. © 2012 The Society for Biotechnology, Japan.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.jbiosc.2012.10.004
dc.sourceScopus
dc.subjectCardiomyocyte
dc.subjectDifferentiation
dc.subjectEmbryonic stem cells
dc.subjectExtracellular matrix
dc.subjectMechanobiology
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1016/j.jbiosc.2012.10.004
dc.description.sourcetitleJournal of Bioscience and Bioengineering
dc.description.volume115
dc.description.issue3
dc.description.page320-325
dc.description.codenJBBIF
dc.identifier.isiut000317456900015
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