Please use this identifier to cite or link to this item: https://doi.org/10.1016/S0378-1097(99)00403-6
DC FieldValue
dc.titleGFP-aided confocal laser scanning microscopy can monitor Agrobacterium tumefaciens cell morphology and gene expression associated with infection
dc.contributor.authorLi, L.
dc.contributor.authorLi, Y.
dc.contributor.authorLim, T.M.
dc.contributor.authorPan, S.Q.
dc.date.accessioned2014-10-27T08:29:29Z
dc.date.available2014-10-27T08:29:29Z
dc.date.issued1999-10-01
dc.identifier.citationLi, L., Li, Y., Lim, T.M., Pan, S.Q. (1999-10-01). GFP-aided confocal laser scanning microscopy can monitor Agrobacterium tumefaciens cell morphology and gene expression associated with infection. FEMS Microbiology Letters 179 (1) : 141-146. ScholarBank@NUS Repository. https://doi.org/10.1016/S0378-1097(99)00403-6
dc.identifier.issn03781097
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/100768
dc.description.abstractWe tagged Agrobacterium tumefaciens cells with a mini-Tn5 transposon containing a promoterless gene encoding a green fluorescent protein (GFP). Some of the GFP-tagged individual bacterial cells exhibited strong green fluorescence, which reflected the expression levels of the GFP-tagged genes. Those cells could be readily detected with a confocal laser scanning microscope (CLSM). We observed that the fluorescence and morphology of A. tumefaciens cells grown in plant tissues resembled those grown in a minimal medium of low pH, which is required for expression of the virulence genes responsible for tumorigenesis. This suggests that GFP-aided CLSM can be used to determine which growth medium is more representative of the nutritional conditions that a pathogen encounters in plant tissues. We also observed that the fluorescence and morphology of A. tumefaciens cells changed dramatically during the course of infection. Our data suggested that A. tumefaciens cells were probably better fed upon successful colonization. We believe that GFP-aided CLSM can help study the fate of A. tumefaciens cells inside plant tissues by monitoring cell morphology and gene expression associated with the infection process in situ. Copyright (C) 1999 Federation of European Microbiological Societies.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/S0378-1097(99)00403-6
dc.sourceScopus
dc.subjectAgrobacterium
dc.subjectConfocal microscope
dc.subjectGreen fluorescent protein
dc.subjectIn situ
dc.subjectInfection
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1016/S0378-1097(99)00403-6
dc.description.sourcetitleFEMS Microbiology Letters
dc.description.volume179
dc.description.issue1
dc.description.page141-146
dc.description.codenFMLED
dc.identifier.isiut000082741600020
Appears in Collections:Staff Publications

Show simple item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

17
checked on Oct 11, 2019

WEB OF SCIENCETM
Citations

15
checked on Oct 11, 2019

Page view(s)

38
checked on Oct 12, 2019

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.