Please use this identifier to cite or link to this item: https://doi.org/10.1007/s10126-002-0007-z
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dc.titleGenetic diversity within and among feral populations and domesticated strains of the guppy (Poecilia reticulata) in Singapore
dc.contributor.authorKhoo, G.
dc.contributor.authorLim, K.F.
dc.contributor.authorGan, D.K.Y.
dc.contributor.authorChen, F.
dc.contributor.authorChan, W.-K.
dc.contributor.authorLim, T.M.
dc.contributor.authorPhang, V.P.E.
dc.date.accessioned2014-10-27T08:29:18Z
dc.date.available2014-10-27T08:29:18Z
dc.date.issued2002-07
dc.identifier.citationKhoo, G., Lim, K.F., Gan, D.K.Y., Chen, F., Chan, W.-K., Lim, T.M., Phang, V.P.E. (2002-07). Genetic diversity within and among feral populations and domesticated strains of the guppy (Poecilia reticulata) in Singapore. Marine Biotechnology 4 (4) : 367-378. ScholarBank@NUS Repository. https://doi.org/10.1007/s10126-002-0007-z
dc.identifier.issn14362228
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/100750
dc.description.abstractGenetic variability within and among feral populations and cultured strains of the guppy (Poecilia reticulata) was investigated by random amplification of polymorphic DNA (RAPD) fingerprinting. Feral guppies were collected from 6 isolated populations (BT, Bukit Timah; NS, Nee Soon; TS, Tuas; MF, Mount Faber; KR, Kranji; LI, laboratory-inbred feral line), while the Tuxedo and Green Variegated strains were sampled from 2 guppy farms in Singapore. Pairwise genetic distances analyzed by unweighted pair-group method with arithmetic means revealed distinct clustering of guppy individuals into their respective populations and strains. Percentage polymorphic loci ranged from 54.96% (TS) to 68.70% (KR), while average heterozygosity ranged from 0.220 (GV) to 0.271 (KR). In contrast, TS guppies had the highest (0.850) intrapopulation genetic similarity (S), whereas KR had the lowest (0.781). Among populations and strains, S ranged from 0.703 (between GV and LI) to 0.809 (between NS and MB). The GV strain S was closer to TX (0.784) than to the feral guppies. Bootstrapped genetic distance trees depicted 3 major nodes comprising BT-TS, NS-MF, and TX-GV. Principal coordinate analysis also differentiated the 6 feral populations from the 2 cultured strains.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1007/s10126-002-0007-z
dc.sourceScopus
dc.subjectCluster analysis
dc.subjectGenetic differentiation
dc.subjectGuppy
dc.subjectPoecilia reticulata
dc.subjectRAPD-PCR
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1007/s10126-002-0007-z
dc.description.sourcetitleMarine Biotechnology
dc.description.volume4
dc.description.issue4
dc.description.page367-378
dc.description.codenMABIF
dc.identifier.isiut000177791100003
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