Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/100639
Title: Expression of a UDPglucose pyrophosphorylase cDNA during fruit ripening of banana (Musa acuminata)
Authors: Pua, E.-C. 
Szu-Wei Lim, S.
Liu, P.
Liu, J.-Z. 
Keywords: Banana
CDNA cloning
Fruit ripening
Musa acuminata
UDPglucose pyrophosphorylase
Issue Date: 2000
Citation: Pua, E.-C.,Szu-Wei Lim, S.,Liu, P.,Liu, J.-Z. (2000). Expression of a UDPglucose pyrophosphorylase cDNA during fruit ripening of banana (Musa acuminata). Australian Journal of Plant Physiology 27 (12) : 1151-1159. ScholarBank@NUS Repository.
Abstract: We report the isolation of a banana cDNA, designated MWUGPA, encoding uridine diphosphoryl (UDP)-glucose pyrophosphorylase (UGPase, EC.2.7.7.9) that catalyses the reversible conversion between glucose 1-phosphate and UDPglucose in plants and animals. Furthermore, UGPase expression in fruit during ripening and in response to exogenous ethylene and sugars was also investigated. MWUGPA encodes a polypeptide of 467 amino acid residues and shares a high degree of sequence similarity (85-90%) with other plant UGPase homologs. In northern blot analysis, a 1.7-kb UGPase transcript was detected in both the vegetative and reproductive organs, but the former was considerably less abundant than the latter. In fruit, the level of accumulated transcripts was higher in pulp than peel at all ripening stages. Transcript abundance in both fruit tissues was relatively constant during ripening, but pulp transcripts surged in the 'more green than yellow' category fruit when ethylene also increased. Further analysis revealed that UGPase expression in fruit was ethylene-inducible, but the response was tissue-specific, as evidenced by the promoting effect of exogenous ethylene on accumulation of UGPase transcripts in pulp but not peel. Exogenous application of sucrose and fructose also increased UGPase transcript abundance in leaf and fruit tissues, especially pulp, whereas exogenous glucose had little or no effect. The results of this study indicate that ethylene and soluble sugars may play a regulatory role in UGPase expression during ripening in banana fruit.
Source Title: Australian Journal of Plant Physiology
URI: http://scholarbank.nus.edu.sg/handle/10635/100639
ISSN: 03107841
Appears in Collections:Staff Publications

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