Please use this identifier to cite or link to this item: https://doi.org/10.1002/cbic.201100798
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dc.titleEstablishing a New Methodology for Genome Mining and Biosynthesis of Polyketides and Peptides through Yeast Molecular Genetics
dc.contributor.authorIshiuchi, K.
dc.contributor.authorNakazawa, T.
dc.contributor.authorOokuma, T.
dc.contributor.authorSugimoto, S.
dc.contributor.authorSato, M.
dc.contributor.authorTsunematsu, Y.
dc.contributor.authorIshikawa, N.
dc.contributor.authorNoguchi, H.
dc.contributor.authorHotta, K.
dc.contributor.authorMoriya, H.
dc.contributor.authorWatanabe, K.
dc.date.accessioned2014-10-27T08:27:40Z
dc.date.available2014-10-27T08:27:40Z
dc.date.issued2012-04
dc.identifier.citationIshiuchi, K., Nakazawa, T., Ookuma, T., Sugimoto, S., Sato, M., Tsunematsu, Y., Ishikawa, N., Noguchi, H., Hotta, K., Moriya, H., Watanabe, K. (2012-04). Establishing a New Methodology for Genome Mining and Biosynthesis of Polyketides and Peptides through Yeast Molecular Genetics. ChemBioChem 13 (6) : 846-854. ScholarBank@NUS Repository. https://doi.org/10.1002/cbic.201100798
dc.identifier.issn14394227
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/100603
dc.description.abstractFungal genome sequencing has revealed many genes coding for biosynthetic enzymes, including polyketide synthases and nonribosomal peptide synthetases. However, characterizing these enzymes and identifying the compounds they synthesize remains a challenge, whether the genes are expressed in their original hosts or in more tractable heterologous hosts, such as yeast. Here, we developed a streamlined method for isolating biosynthetic genes from fungal sources and producing bioactive molecules in an engineered Saccharomyces cerevisiae host strain. We used overlap extension PCR and yeast homologous recombination to clone desired fungal polyketide synthase or a nonribosomal peptide synthetase genes (5-20 kb) into a yeast expression vector quickly and efficiently. This approach was used successfully to clone five polyketide synthases and one nonribosomal peptide synthetase, from various fungal species. Subsequent detailed chemical characterizations of the resulting natural products identified six polyketide and two nonribosomal peptide products, one of which was a new compound. Our system should facilitate investigating uncharacterized fungal biosynthetic genes, identifying novel natural products, and rationally engineering biosynthetic pathways for the production of enzyme analogues possessing modified bioactivity. © 2012 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1002/cbic.201100798
dc.sourceScopus
dc.subjectDrug discovery
dc.subjectFungal putative gene cluster
dc.subjectNonribosomal peptide synthetase
dc.subjectPolyketides
dc.subjectYeast heterologous biosynthesis
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1002/cbic.201100798
dc.description.sourcetitleChemBioChem
dc.description.volume13
dc.description.issue6
dc.description.page846-854
dc.description.codenCBCHF
dc.identifier.isiut000302609100014
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