Please use this identifier to cite or link to this item: https://doi.org/10.1242/dev.082362
DC FieldValue
dc.titleDynamic microtubules at the vegetal cortex predict the embryonic axis in zebrafish
dc.contributor.authorTran, L.D.
dc.contributor.authorHino, H.
dc.contributor.authorQuach, H.
dc.contributor.authorLim, S.
dc.contributor.authorShindo, A.
dc.contributor.authorMimori-Kiyosue, Y.
dc.contributor.authorMione, M.
dc.contributor.authorUeno, N.
dc.contributor.authorWinkler, C.
dc.contributor.authorHibi, M.
dc.contributor.authorSampath, K.
dc.date.accessioned2014-10-27T08:26:41Z
dc.date.available2014-10-27T08:26:41Z
dc.date.issued2012-10-01
dc.identifier.citationTran, L.D., Hino, H., Quach, H., Lim, S., Shindo, A., Mimori-Kiyosue, Y., Mione, M., Ueno, N., Winkler, C., Hibi, M., Sampath, K. (2012-10-01). Dynamic microtubules at the vegetal cortex predict the embryonic axis in zebrafish. Development (Cambridge) 139 (19) : 3644-3652. ScholarBank@NUS Repository. https://doi.org/10.1242/dev.082362
dc.identifier.issn09501991
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/100512
dc.description.abstractIn zebrafish, as in many animals, maternal dorsal determinants are vegetally localized in the egg and are transported after fertilization in a microtubule-dependent manner. However, the organization of early microtubules, their dynamics and their contribution to axis formation are not fully understood. Using live imaging, we identified two populations of microtubules, perpendicular bundles and parallel arrays, which are directionally oriented and detected exclusively at the vegetal cortex before the first cell division. Perpendicular bundles emanate from the vegetal cortex, extend towards the blastoderm, and orient along the animal-vegetal axis. Parallel arrays become asymmetric on the vegetal cortex, and orient towards dorsal. We show that the orientation of microtubules at 20 minutes post-fertilization can predict where the embryonic dorsal structures in zebrafish will form. Furthermore, we find that parallel microtubule arrays colocalize with wnt8a RNA, the candidate maternal dorsal factor. Vegetal cytoplasmic granules are displaced with parallel arrays by ~20°, providing in vivo evidence of a cortical rotation-like process in zebrafish. Cortical displacement requires parallel microtubule arrays, and probably contributes to asymmetric transport of maternal determinants. Formation of parallel arrays depends on Ca2+ signaling. Thus, microtubule polarity and organization predicts the zebrafish embryonic axis. In addition, our results suggest that cortical rotation-like processes might be more common in early development than previously thought.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1242/dev.082362
dc.sourceScopus
dc.subjectCortical rotation
dc.subjectDorsoventral patterning
dc.subjectEmbryonic axes
dc.subjectMicrotubule dynamics
dc.subjectPolarity
dc.subjectwnt8a RNA
dc.subjectZebrafish
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1242/dev.082362
dc.description.sourcetitleDevelopment (Cambridge)
dc.description.volume139
dc.description.issue19
dc.description.page3644-3652
dc.description.codenDEVPE
dc.identifier.isiut000308391400018
Appears in Collections:Staff Publications

Show simple item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

39
checked on Jan 14, 2020

WEB OF SCIENCETM
Citations

39
checked on Jan 14, 2020

Page view(s)

80
checked on Jan 11, 2020

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.