Please use this identifier to cite or link to this item: https://doi.org/10.1242/dev.077081
Title: Dorsal activity of maternal squint is mediated by a noncoding function of the RNA
Authors: Lim, S.
Kumari, P.
Gilligan, P.
Quach, H.N.B.
Mathavan, S.
Sampath, K. 
Keywords: 3'UTR
Axis formation
Dorsal expansion
Dorsal localization
Maternal factors
Nodal
Non-coding RNA
RNA localization
Squint (nodal-related 1)
Zebrafish
Issue Date: 15-Aug-2012
Citation: Lim, S., Kumari, P., Gilligan, P., Quach, H.N.B., Mathavan, S., Sampath, K. (2012-08-15). Dorsal activity of maternal squint is mediated by a noncoding function of the RNA. Development (Cambridge) 139 (16) : 2903-2915. ScholarBank@NUS Repository. https://doi.org/10.1242/dev.077081
Abstract: Despite extensive study, the earliest steps of vertebrate axis formation are only beginning to be elucidated. We previously showed that asymmetric localization of maternal transcripts of the conserved zebrafish TGFβ factor Squint (Sqt) in 4-cell stage embryos predicts dorsal, preceding nuclear accumulation of β-catenin. Cell ablations and antisense oligonucleotides that deplete Sqt lead to dorsal deficiencies, suggesting that localized maternal sqt functions in dorsal specification. However, based upon analysis of sqt and Nodal signaling mutants, the function and mechanism of maternal sqt was debated. Here, we show that sqt RNA may function independently of Sqt protein in dorsal specification. sqt insertion mutants express localized maternal sqt RNA. Overexpression of mutant/non-coding sqt RNA and, particularly, the sqt 3'UTR, leads to ectopic nuclear β-catenin accumulation and expands dorsal gene expression. Dorsal activity of sqt RNA requires Wnt/β-catenin but not Oep-dependent Nodal signaling. Unexpectedly, sqt ATG morpholinos block both sqt RNA localization and translation and abolish nuclear β-catenin, providing a mechanism for the loss of dorsal identity in sqt morphants and placing maternal sqt RNA upstream of β-catenin. The loss of early dorsal gene expression can be rescued by the sqt 3'UTR. Our findings identify new non-coding functions for the Nodal genes and support a model wherein sqt RNA acts as a scaffold to bind and deliver/sequester maternal factors to future embryonic dorsal. © 2012.
Source Title: Development (Cambridge)
URI: http://scholarbank.nus.edu.sg/handle/10635/100503
ISSN: 09501991
DOI: 10.1242/dev.077081
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

19
checked on Dec 7, 2019

WEB OF SCIENCETM
Citations

20
checked on Nov 29, 2019

Page view(s)

56
checked on Nov 30, 2019

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.