Please use this identifier to cite or link to this item:
|Title:||Crystallization of a nonclassical Kazal-type Carcinoscorpius rotundicauda serine protease inhibitor, CrSPI-1, complexed with subtilisin||Authors:||Tulsidas, S.R.
Kazal-type serine protease inhibitors
|Issue Date:||2009||Citation:||Tulsidas, S.R., Thangamani, S., Ho, B., Sivaraman, J., Ding, J.L. (2009). Crystallization of a nonclassical Kazal-type Carcinoscorpius rotundicauda serine protease inhibitor, CrSPI-1, complexed with subtilisin. Acta Crystallographica Section F: Structural Biology and Crystallization Communications 65 (5) : 533-535. ScholarBank@NUS Repository. https://doi.org/10.1107/S1744309109014420||Abstract:||Serine proteases play a major role in host-pathogen interactions. The innate immune system is known to respond to invading pathogens in a nonspecific manner. The serine protease cascade is an essential component of the innate immune system of the horseshoe crab. The serine protease inhibitor CrSPI isoform 1 (CrSPI-1), a unique nonclassical Kazal-type inhibitor of molecular weight 9.3 kDa, was identified from the hepatopancreas of the horseshoe crab Carcinoscorpius rotundicauda. It potently inhibits subtilisin and constitutes a powerful innate immune defence against invading microbes. Here, the cloning, expression, purification and cocrystallization of CrSPI-1 with subtilisin are reported. The crystals diffracted to 2.6 Å resolution and belonged to space group P21, with unit-cell parameters a = 73.8, b = 65.0, c = 111.9 Å, Β = 95.4°. The Matthews coefficient (V M = 2.64 Å3 Da-1, corresponding to 53% solvent content) and analysis of the preliminary structure solution indicated the presence of one heterotrimer (1:2 ratio of CrSPI-1:subtilisin) and one free subtilisin molecule in the asymmetric unit. © 2009 International Union of Crystallography. All right reserved.||Source Title:||Acta Crystallographica Section F: Structural Biology and Crystallization Communications||URI:||http://scholarbank.nus.edu.sg/handle/10635/100377||ISSN:||17443091||DOI:||10.1107/S1744309109014420|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Oct 26, 2020
WEB OF SCIENCETM
checked on Oct 26, 2020
checked on Oct 10, 2020
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.