Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.fsi.2003.10.003
DC FieldValue
dc.titleCloning, characterisation and expression of Aeromonas hydrophila major adhesin
dc.contributor.authorFang, H.-M.
dc.contributor.authorGe, R.
dc.contributor.authorSin, Y.M.
dc.date.accessioned2014-10-27T08:24:06Z
dc.date.available2014-10-27T08:24:06Z
dc.date.issued2004-05
dc.identifier.citationFang, H.-M., Ge, R., Sin, Y.M. (2004-05). Cloning, characterisation and expression of Aeromonas hydrophila major adhesin. Fish and Shellfish Immunology 16 (5) : 645-658. ScholarBank@NUS Repository. https://doi.org/10.1016/j.fsi.2003.10.003
dc.identifier.issn10504648
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/100285
dc.description.abstractAeromonas hydrophila, an important pathogen in fish, is believed to cause diseases by adhesive and enterotoxic mechanisms. The adhesion is a prerequisite for successful invasion. In this study, the gene of a 43 kDa major adhesin (designated as AHA1) was cloned and expressed. Nucleotide sequence analysis of AHA1 revealed an open reading frame encoding a polypeptide of 373 amino acids with a 20-amino-acid putative signal peptide (molecular weight 40,737 Da). The amino acid sequences of Aha1p showed a very high homology with the other two outer membrane proteins of A. hydrophila. Using the T-5 expression system, this major adhesin Aha1p was expressed in Escherichia coli. The purified recombinant adhesin could competitively inhibit A. hydrophila from invading fish epithelial cells in vitro. Western-blot analysis showed that this major adhesin is a very conserved antigen among various strains of Aeromonas. When used to immunise blue gourami, the recombinant adhesin could confer significant protection to fish against experimental A. hydrophila challenge. © 2004 Elsevier Ltd. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.fsi.2003.10.003
dc.sourceScopus
dc.subjectA. hydrophila
dc.subjectAdhesin
dc.subjectImmunisation
dc.subjectOuter membrane protein
dc.subjectProtection
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1016/j.fsi.2003.10.003
dc.description.sourcetitleFish and Shellfish Immunology
dc.description.volume16
dc.description.issue5
dc.description.page645-658
dc.description.codenFSIME
dc.identifier.isiut000221496900009
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