Please use this identifier to cite or link to this item: https://doi.org/10.1073/pnas.0504666102
DC FieldValue
dc.titleCloning of a human parvovirus by molecular screening of respiratory tract samples
dc.contributor.authorAllander, T.
dc.contributor.authorTammi, M.T.
dc.contributor.authorEriksson, M.
dc.contributor.authorBjerkner, A.
dc.contributor.authorTiveljung-Lindell, A.
dc.contributor.authorAndersson, B.
dc.date.accessioned2014-10-27T08:24:04Z
dc.date.available2014-10-27T08:24:04Z
dc.date.issued2005-09-06
dc.identifier.citationAllander, T., Tammi, M.T., Eriksson, M., Bjerkner, A., Tiveljung-Lindell, A., Andersson, B. (2005-09-06). Cloning of a human parvovirus by molecular screening of respiratory tract samples. Proceedings of the National Academy of Sciences of the United States of America 102 (36) : 12891-12896. ScholarBank@NUS Repository. https://doi.org/10.1073/pnas.0504666102
dc.identifier.issn00278424
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/100282
dc.description.abstractThe identification of new virus species is a key issue for the study of infectious disease but is technically very difficult. We developed a system for large-scale molecular virus screening of clinical samples based on host DNA depletion, random PCR amplification, large-scale sequencing, and bioinformatics. The technology was applied to pooled human respiratory tract samples. The first experiments detected seven human virus species without the use of any specific reagent. Among the detected viruses were one coronavirus and one parvovirus, both of which were at that time uncharacterized. The parvovirus, provisionally named human bocavirus, was in a retrospective clinical study detected in 17 additional patients and associated with lower respiratory tract infections in children. The molecular virus screening procedure provides a general culture-independent solution to the problem of detecting unknown virus species in single or pooled samples. We suggest that a systematic exploration of the viruses that infect humans, "the human virome," can be initiated. © 2005 by The National Academy of Sciences of the USA.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1073/pnas.0504666102
dc.sourceScopus
dc.subjectBioinformatics
dc.subjectNucleotide sequencing
dc.subjectRespiratory tract infection
dc.subjectVirus
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1073/pnas.0504666102
dc.description.sourcetitleProceedings of the National Academy of Sciences of the United States of America
dc.description.volume102
dc.description.issue36
dc.description.page12891-12896
dc.description.codenPNASA
dc.identifier.isiut000231716700045
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