Please use this identifier to cite or link to this item: https://doi.org/10.1016/S0145-305X(00)00052-5
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dc.titleCloning and developmental expression of a family of pleurocidin-like antimicrobial peptides from winter flounder, Pleuronectes americanus (Walbaum)
dc.contributor.authorDouglas, S.E.
dc.contributor.authorGallant, J.W.
dc.contributor.authorGong, Z.
dc.contributor.authorHew, C.
dc.date.accessioned2014-10-27T08:24:01Z
dc.date.available2014-10-27T08:24:01Z
dc.date.issued2001
dc.identifier.citationDouglas, S.E., Gallant, J.W., Gong, Z., Hew, C. (2001). Cloning and developmental expression of a family of pleurocidin-like antimicrobial peptides from winter flounder, Pleuronectes americanus (Walbaum). Developmental and Comparative Immunology 25 (2) : 137-147. ScholarBank@NUS Repository. https://doi.org/10.1016/S0145-305X(00)00052-5
dc.identifier.issn0145305X
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/100278
dc.description.abstractLow molecular weight antimicrobial peptides are an important component of the innate immune system in animals, yet they have not been examined widely in fish. Of particular interest is their expression during development and in response to environmental conditions and disease. Here, we report the isolation of four genomic sequences encoding putative antimicrobial peptides from the winter flounder, Pleuronectes americanus (Walbaum), as well as reverse transcription-PCR products from two tissues that form the first defensive barrier to microbes - skin and intestine. Alignment of the predicted polypeptide sequences shows a conserved hydrophobic signal peptide of 22 amino acids followed by 25 amino acids that are identical (WF2) or homologous to the amino acid sequence of pleurocidin, followed by a conserved acidic portion. Southern hybridisation analysis indicates that related peptides are encoded in the genomes of other flatfish species. Northern and RT-PCR analyses of RNA from multiple tissues show that two of the pleurocidin genes are expressed predominantly in the skin whereas two other genes are expressed mainly in the intestine. RT-PCR assays of total RNA from larvae of different ages provide the first evidence of developmental expression of antimicrobial peptides in fish and indicate that the pleurocidin gene is first expressed at 13 days post-hatch in winter flounder. Copyright (C) 2000 .
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/S0145-305X(00)00052-5
dc.sourceScopus
dc.subjectAntimicrobial peptide
dc.subjectDevelopment
dc.subjectExpression
dc.subjectGene structure
dc.subjectInnate immunity
dc.subjectPleuronectes americanus
dc.subjectPluerocidin
dc.subjectReverse transcription-PCR (RT-PCR)
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1016/S0145-305X(00)00052-5
dc.description.sourcetitleDevelopmental and Comparative Immunology
dc.description.volume25
dc.description.issue2
dc.description.page137-147
dc.description.codenDCIMD
dc.identifier.isiut000165962600004
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