Please use this identifier to cite or link to this item: https://doi.org/10.1016/S0378-1119(03)00605-X
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dc.titleCloning and characterization of rice HMGB1 gene
dc.contributor.authorWu, Q.
dc.contributor.authorZhang, W.
dc.contributor.authorPwee, K.-H.
dc.contributor.authorKumar, P.P.
dc.date.accessioned2014-10-27T08:23:59Z
dc.date.available2014-10-27T08:23:59Z
dc.date.issued2003-07-17
dc.identifier.citationWu, Q., Zhang, W., Pwee, K.-H., Kumar, P.P. (2003-07-17). Cloning and characterization of rice HMGB1 gene. Gene 312 (1-2) : 103-109. ScholarBank@NUS Repository. https://doi.org/10.1016/S0378-1119(03)00605-X
dc.identifier.issn03781119
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/100275
dc.description.abstractWe isolated a 918 bp long full-length rice HMGB1 cDNA, which has an open reading frame of 471 bp encoding 157 amino acids, with a central domain of high sequence similarity to the HMG-box domain of other plant HMGB1 proteins. RNA gel blot analysis indicated that rice HMGB1 gene is constitutively expressed in various tissues and organs. Southern hybridization and sequence analyses suggested that a single copy of the HMGB1 gene composed of seven exons and six introns exists in rice. We have also cloned a 1755 bp long 5′ flanking region of the rice HMGB1 gene, which can be regarded as its promoter. 5′ deletion analysis of this promoter indicated that positive cis-elements residing between -1400 and -1115 are important to enhance quantitative expression, whereas negative cis-elements between -1755 and -1400 and between -1115 and -351 inhibit expression. © 2003 Elsevier Science B.V. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/S0378-1119(03)00605-X
dc.sourceScopus
dc.subjectcis-elements
dc.subjectGUS reporter gene expression
dc.subjectPromoter deletion
dc.subjectRice HMGB1 cDNA
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1016/S0378-1119(03)00605-X
dc.description.sourcetitleGene
dc.description.volume312
dc.description.issue1-2
dc.description.page103-109
dc.description.codenGENED
dc.identifier.isiut000184967900010
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