Please use this identifier to cite or link to this item: https://doi.org/10.1016/S0378-1119(02)01151-4
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dc.titleCloning and characterization of a metalloprotease from Vibrio harveyi strain AP6
dc.contributor.authorTeo, J.W.P.
dc.contributor.authorZhang, L.-H.
dc.contributor.authorPoh, C.L.
dc.date.accessioned2014-10-27T08:23:56Z
dc.date.available2014-10-27T08:23:56Z
dc.date.issued2003-01-16
dc.identifier.citationTeo, J.W.P., Zhang, L.-H., Poh, C.L. (2003-01-16). Cloning and characterization of a metalloprotease from Vibrio harveyi strain AP6. Gene 303 (1-2) : 147-156. ScholarBank@NUS Repository. https://doi.org/10.1016/S0378-1119(02)01151-4
dc.identifier.issn03781119
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/100270
dc.description.abstractA metalloprotease gene pap6 was cloned from Vibrio harveyi strain AP6. Sequence analysis showed that pap6 was 2034 bp in length and predicted to encode a peptide of 677 amino acids with a molecular mass of 75 kDa. SDS-PAGE analysis of the purified Pap6 revealed that it was 35 kDa in size. N-terminal amino acid sequencing established that the mature protein began at Leu-191, suggesting that the preprotein of Pap6 was processed to generate a mature protease. Purified Pap6 was characterized as a zinc metalloprotease as it was inhibited by zinc- and metal-specific inhibitors such as 1, 10-phenanthroline, EGTA and EDTA. The deduced amino acid sequence revealed the presence of a zinc-binding motif HEXXH∼19aa∼E. Substitution of these active site residues by site-directed mutagenesis caused significant losses in enzyme activity, thus demonstrating their involvement in catalysis. Pap6 was shown to digest a range of host proteins, including gelatin, fibronectin, and type IV collagen, indicating a potential role in pathogenesis. © 2002 Elsevier Science B.V. All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/S0378-1119(02)01151-4
dc.sourceScopus
dc.subjectPathogenesis
dc.subjectZinc metalloprotease
dc.subjectZinc-binding motif
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1016/S0378-1119(02)01151-4
dc.description.sourcetitleGene
dc.description.volume303
dc.description.issue1-2
dc.description.page147-156
dc.description.codenGENED
dc.identifier.isiut000181124200015
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