Please use this identifier to cite or link to this item:
|Title:||Carbon nanotube-based labels for highly sensitive colorimetric and aggregation-based visual detection of nucleic acids||Authors:||Lee, A.C.
|Issue Date:||14-Nov-2007||Citation:||Lee, A.C., Ye, J.-S., Tan, S.N., Poenar, D.P., Sheu, F.-S., Heng, C.K., Lim, T.M. (2007-11-14). Carbon nanotube-based labels for highly sensitive colorimetric and aggregation-based visual detection of nucleic acids. Nanotechnology 18 (45) : -. ScholarBank@NUS Repository. https://doi.org/10.1088/0957-4484/18/45/455102||Abstract:||A novel carbon nanotube (CNT) derived label capable of dramatic signal amplification of nucleic acid detection and direct visual detection of target hybridization has been developed. Highly sensitive colorimetric detection of human acute lymphocytic leukemia (ALL) related oncogene sequences amplified by the novel CNT-based label was demonstrated. Atomic force microscope (AFM) images confirmed that a monolayer of horseradish peroxidase and detection probe molecules was immobilized along the carboxylated CNT carrier. The resulting CNT labels significantly enhanced the nucleic acid assay sensitivity by at least 1000 times compared to that of conventional labels used in enzyme-linked oligosorbent assay (ELOSA). An excellent detection limit of 1 × 10 -12 M (60 × 10-18 mol in 60 μl) and a four-order wide dynamic range of target concentration were achieved. Hybridizations using these labels were coupled to a concentration-dependent formation of visible dark aggregates. Targets can thus be detected simply with visual inspection, eliminating the need for expensive and sophisticated detection systems. The approach holds promise for ultrasensitive and low cost visual inspection and colorimetric nucleic acid detection in point-of-care and early disease diagnostic application. © IOP Publishing Ltd.||Source Title:||Nanotechnology||URI:||http://scholarbank.nus.edu.sg/handle/10635/100214||ISSN:||09574484||DOI:||10.1088/0957-4484/18/45/455102|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on May 18, 2020
WEB OF SCIENCETM
checked on May 18, 2020
checked on May 10, 2020
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.