Please use this identifier to cite or link to this item: https://doi.org/10.1016/j.ymthe.2004.08.018
DC FieldValue
dc.titleAxonal transport of recombinant baculovirus vectors
dc.contributor.authorLi, Y.
dc.contributor.authorWang, X.
dc.contributor.authorGuo, H.
dc.contributor.authorWang, S.
dc.date.accessioned2014-10-27T08:22:33Z
dc.date.available2014-10-27T08:22:33Z
dc.date.issued2004-12
dc.identifier.citationLi, Y., Wang, X., Guo, H., Wang, S. (2004-12). Axonal transport of recombinant baculovirus vectors. Molecular Therapy 10 (6) : 1121-1129. ScholarBank@NUS Repository. https://doi.org/10.1016/j.ymthe.2004.08.018
dc.identifier.issn15250016
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/100143
dc.description.abstractTargeted gene delivery to neurons is crucial to effective gene therapy of neurodegenerative diseases. Several types of viral gene vectors may target neurons through retrograde axonal transport to somas of projection neurons after viral internalization at axon terminal fields. In this report we demonstrate for the first time that recombinant baculovirus vectors could migrate by axonal transport to cell bodies, resulting in transgene expression in projection neurons. After stereotaxic injection of Cy3-labeled baculovirus vectors into the rat striatum, retrograde axonal transport of the baculovirus vectors was observed along the corticostriatal pathway and nigrostriatal pathway. Furthermore, after intra-vitreous body injection, anterograde axonal transport and transsynaptic transport of the virus particles were observed in defined connections of the visual system, from the retina to the optic nerve, the lateral geniculate body, the superior colliculus, and the primary visual cortex. PCR analysis confirmed the existence of transported viral DNA in the tissue samples collected from projection fields. Driven by a neuron-specific promoter, transgene expression from the recombinant baculovirus vectors was detectable in target regions remote from injection sites. The attributes of baculovirus vectors in the bidirectional axonal transport and transneuronal transport in neural circuits of the central nervous system could be utilized for targeted gene delivery. Copyright © The American Society of Gene Therapy.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/j.ymthe.2004.08.018
dc.sourceScopus
dc.subjectAxonal transport
dc.subjectBaculovirus
dc.subjectGene delivery
dc.subjectNeuron
dc.typeArticle
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1016/j.ymthe.2004.08.018
dc.description.sourcetitleMolecular Therapy
dc.description.volume10
dc.description.issue6
dc.description.page1121-1129
dc.description.codenMTOHC
dc.identifier.isiut000226001000016
Appears in Collections:Staff Publications

Show simple item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

48
checked on Jul 2, 2020

WEB OF SCIENCETM
Citations

45
checked on Jun 24, 2020

Page view(s)

81
checked on Jun 27, 2020

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.