Application of zebrafish microarray on the toxicity mechanism study of bisphenol A

Abstract

The toxicity mechanism of bisphenol A (BPA) to zebrafish (Danio rerio) was studied in the molecular level, by the method of zebrafish microarray and quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR). Zebrafish embryos were exposed to 0.5, 1.5 and 4.5 mg/L BPA for 8 days since fertilization. The results from microarray and validated by qRT-PCR showed that, 50 specific genes were up-or down-regulated, and dose-responses for them were significant (p < 0.05). This study demonstrates the genotoxicity of BPA. Moreover, according to their function and pathway analysis, it could support the mechanisms for the morphological toxicity and metabolize turbulence observed in prophase study.