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dc.titleAn Agrobacterium gene involved in tumorigenesis encodes an outer membrane protein exposed on the bacterial cell surface
dc.contributor.authorJia, Y.H.
dc.contributor.authorLi, L.P.
dc.contributor.authorHou, Q.M.
dc.contributor.authorPan, S.Q.
dc.identifier.citationJia, Y.H., Li, L.P., Hou, Q.M., Pan, S.Q. (2002-02-06). An Agrobacterium gene involved in tumorigenesis encodes an outer membrane protein exposed on the bacterial cell surface. Gene 284 (1-2) : 113-124. ScholarBank@NUS Repository.
dc.description.abstractA gene designated as aopB was identified which was involved in tumorigenesis of Agrobacterium tumefaciens. aopB is located on the circular chromosome as a single copy. This gene shares high homology with ropB, a Rhizobium leguminosarum gene encoding an outer membrane protein. A transposon mutant CGI1 containing a gfp-tagged transposon insertion at aopB caused attenuated tumors on plants when inoculated at a low cell concentration (5 × 107 cells/ml). The mutation did not affect the bacterial growth on different media. A broad host range plasmid containing the wild type aopB could restore the tumor formation ability of CGI1 to the wild type level. When both aopB-gfp and aopB-phoA fusions were used to study the aopB gene expression, we found that the aopB gene was inducible by acidic pH but not by plant phenolic compound acetosyringone. aopB encodes a putative protein of 218 amino acids with a predicted molecular weight of 22.8 kDa. TnphoA transposon mutagenesis of aopB, subcellular fractionation and whole cell ELISA experiments indicated that AopB is an outer membrane protein exposed on the bacterial cell surface. It appeared that AopB was exclusively present in the outer membrane and not in other fractions. The vir gene induction assays showed that the aopB gene was not required for the expression of the Ti plasmid encoded vir genes that are essential for tumorigenesis. The C-terminal half of AopB is slightly homologous to some of the bacterial porin proteins and some of plant dehydrins. The role of AopB in Agrobacterium-plant interaction is discussed. © 2002 Elsevier Science B.V. All rights reserved.
dc.subjectCell surface protein
dc.subjectGreen fluorescent protein
dc.subjectLow pH induction
dc.subjectProtein topology
dc.contributor.departmentBIOLOGICAL SCIENCES
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